FastQCFastQC Report
Tue 25 Jul 2023
SRR9596309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56162410
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT992610.1767392104434265TruSeq Adapter, Index 3 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT875970.15597087090813944TruSeq Adapter, Index 3 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT690880.12301466407869605TruSeq Adapter, Index 3 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)