FastQCFastQC Report
Fri 14 Jul 2023
SRR9601985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9601985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78821592
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT2182406927.687932260997723No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1657294121.025889707987627No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG62199677.8911968690000585No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG21505602.728389449429035No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCT17696712.2451601840267323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCAACTTAGAACTGGTACGGACAGGGGGAAAGATCGGAAGAGCACACG7549150.9577515257494418No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTG7137980.9055868853803409Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACG5125880.6503141930957193No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGT4799640.6089245190581789No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACGT4195530.5322818143536101No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCA4089290.5188032741079374No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGA3486740.4423584847157109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGGCGAGCATGTCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT3272120.41512990501384445No Hit
CAACCAACTTAGAACTGGTACGGACAGGGGGAAAGATCGGAAGAGCACAC3265250.4142583164267984No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACG3183160.40384365745873285No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTC2943170.37339641655550426No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGAGATCGGAAGAGCAC2607350.330791339510118No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACAC2501060.3173064558249471No Hit
TCTGGCGAGCATGTCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC2396190.3040017258215236No Hit
TTCTGGTGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT2356970.2990259318791734No Hit
TTACGATCTGCTGAGGGTAAGCCCTCCTTCGCCTAAGATCGGAAGAGCAC2258940.28658898439909714No Hit
AACCAACTTAGAACTGGTACGGACAGGGGGAATAGATCGGAAGAGCACAC2206750.2799677022509264No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACG2173460.27574424023305694No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACAC1962010.2489178345953733No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACA1876530.23807309042933314No Hit
GATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGT1870250.23727635442836528No Hit
TAATGCCAACAATCCATTTACAACCTAAATAAAAACGAAAAACAAAACTA1743760.22122872118594106No Hit
ATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGTC1663070.21099167852382375No Hit
TAGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGC1588890.2015805516843659No Hit
TTCTGGCGAACATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT1570120.19919922449675972No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCA1545680.19609855127006318No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAAGATCGGAAGAGCAC1393350.17677262849499412No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACAC1392200.17662672938653662No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATG1387820.17607104408649854TruSeq Adapter, Index 25 (100% over 49bp)
TCTGGTGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1373260.17422383450463674No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGC1285930.16314438307716495No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACGT1183850.1501936169977384No Hit
TCTGGCGAACATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1182120.14997413399110232No Hit
TTCTGGCGAGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT1163000.14754840272700911No Hit
TCTGACGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1130610.14343912262010644No Hit
GCCAAGGATGTTTTCATTAATCAGGAACAGATCGGAAGAGCACACGTCTG1128620.14318665372807998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACG1121890.14233282677162876No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGTC1071670.13596147613968518No Hit
TTCTGGCGAGCATGCCTGTTCGAGTGTCATTAGATCGGAAGAGCACACGT1009960.12813240311106633No Hit
CATCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACA994090.12611899541435295No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTAGATCGGAAGAGCAC991340.12577010624195462No Hit
TTCTGGCGAGCATGCCTGTTCGAGCATCATTAGATCGGAAGAGCACACGT981670.12454328504301207No Hit
CTGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCACA978300.12411573722083664No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCT969060.1229434696015782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGCGAGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC949950.12051900702538462No Hit
TTCTGACGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT938700.11909173313830047No Hit
ATTCTGGCGAGCATGTCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG916460.11627017124952259No Hit
ATCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACAC884710.11224208716819625No Hit
TTCTGGCGAGCATGCTTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT823230.10444219396126889No Hit
AACCAACTTAGAACTGGTACGGACAGGGGGAAGATCGGAAGAGCACACGT823190.10443711920967036No Hit
TTCTGGCGAGCATGCCTGTTCAAGCGTCATTAGATCGGAAGAGCACACGT822710.10437622219048812No Hit
TGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCACAC816150.1035439629283306No Hit
TGCAAGGATGCTGGCGTAATGGTCATCAACGACCAGATCGGAAGAGCACA813860.10325343339931525No Hit
ACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTCT809800.10273834611206534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGCAAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT798430.10129584797018563No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGAGATCGGAAGAGCACACGT798290.10127808633959082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)