FastQCFastQC Report
Tue 25 Jul 2023
SRR9601986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9601986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145095480
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC2408813416.601574356416894No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT140132419.657944547962487No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACG62746704.3245110047535595No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACGT49376153.4030109001327955No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCT32777662.259040736486071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG29157432.0095339978888385No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTC23788881.6395328097057194No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCA18697561.2886383504158778No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGA17276101.19067113599955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTG16551161.1407081736798417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCT12443150.8575835718659189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACAC9922630.6838689944028581No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACG9877200.6807379526915656No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGT7802580.5377548632114523No Hit
ACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTCT7310510.5038413326176666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG7222910.49780392883362046No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGTC6992140.48189922939019186No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTAGATCGGAAGAGCAC6620620.456294021012922No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGAGATCGGAAGAGCAC6391190.4404816745497517No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCA5624030.3876089041505635No Hit
TCTGGCGAGCATGTCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC5498440.3789532244560616No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACG5265200.362878292280366No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCT4977470.343047901974617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCAC4893470.3372586106748467No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACG4831260.3329710891062906No Hit
TCTGGTGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC3738470.2576558553030046No Hit
TCTGGCGAACATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC3607790.24864937212379046No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCAGATCGGAAGAGCACACGTCTGA3359300.23152340789664846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGACGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC3355770.23128011982178906No Hit
TTCTGGCGAGCATGTCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT3298380.22732479330162453No Hit
GATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGT3284490.2263674926331268No Hit
ATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGTC3053240.21042971152512813No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACGT3047100.21000654189916876No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGC3022580.20831662020071198No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAAGATCGGAAGAGCACACGT2987530.2059009694857483No Hit
CGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCTG2713820.18703683946598473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGT2659230.18327448932247925No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCAGATCGGAAGAGCACACGTCTG2640110.18195673635043627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTC2636220.1816886370271493No Hit
TTCTGGTGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT2630250.18127718382405847No Hit
TCTGGCGAGCATGCCTGTTCGAGTGTCATTAGATCGGAAGAGCACACGTC2511980.1731259995142509No Hit
CATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGT2502660.17248366386051447No Hit
TAGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGC2449470.1688178019053385No Hit
ATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTC2442850.16836155061480895No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACA2345370.16164321590169453No Hit
TCTGGCGAGCATGCCTGTTCAAGCGTCATTAGATCGGAAGAGCACACGTC2316590.159659694430178No Hit
TAATGCCAACAATCCATTTACAACCTAAATAAAAACGAAAAACAAAACTA2305600.15890226215179135No Hit
TCTGGCGAGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC2240900.1544431294482778No Hit
TCTGGCAAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC2085750.14375017057733294No Hit
TTCTGGCGAACATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT2070000.14266467845862602No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATG2008830.13844883382997183TruSeq Adapter, Index 7 (97% over 37bp)
TCTGGCGAGCATGCCTGTTCGAGCATCATTAGATCGGAAGAGCACACGTC1962530.13525783160164603No Hit
GAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCTG1920760.13237903758270073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGATTCATAATAACTTCTCGAATCGCAGATCGGAAGAGCACACGTC1898780.13086417302592748No Hit
TCTGGCGAGCATGCATGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1821530.1255400926341744No Hit
CATCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACG1777340.12249451188968809No Hit
GTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGTC1759690.1212780715153911No Hit
CTGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCACA1753700.12086523990960986No Hit
GGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACGTC1595700.10997585865527995No Hit
TCTGGCGAGTATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1592840.10977874707054967No Hit
TCTGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCAC1587160.10938728070646997No Hit
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGCA1574330.10850303538056459No Hit
GCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA1508840.10398945577077935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGGCGAGCATGCCTGTTCGAGTGTCATTAGATCGGAAGAGCACACGT1482830.10219684307188619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)