FastQCFastQC Report
Fri 29 Sept 2023
SRR9620586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9620586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232616884
Total Bases11.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41740661.7943951136410203Illumina PCR Primer Index 6 (96% over 29bp)
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC7631450.3280694792558566No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAATA6389690.2746872836625221No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCT5765900.24787108746586078No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTAT4377530.18818625392643468No Hit
ATGAGGTAAAGCGAATGATTAGAGGCCTGTAGGCACCATCAATATCTCGT3961270.1702915941389706No Hit
CCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT3892830.16734941733636152No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA3838650.1650202656828642Illumina PCR Primer Index 6 (96% over 29bp)
CGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCTC3711560.15955677576697314No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATAT3501840.15054109313922373No Hit
ATCGAGTCTTTGAACGCAACTTGCGCCCTTCTGTAGGCACCATCAATATC3287250.14131605339533307No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATA3131330.13461318654754226No Hit
AGTTAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTC3119650.13411107338193043No Hit
TTGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT2971970.12776243705508494No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATC2949070.12677798572867136Illumina PCR Primer Index 6 (96% over 29bp)
TTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTA2893750.12439982645455777No Hit
TTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTATG2568930.1104360937102055No Hit
TGGCTGAGAAGCTGATCAAACTTGGTCATTTCTGTAGGCACCATCAATAT2486500.10689249882652543No Hit
TGGAATGAGTACAATTTAAATCCCTTAACGACTGTAGGCACCATCAATAT2426870.10432905635516983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)