FastQCFastQC Report
Fri 29 Sept 2023
SRR9620588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9620588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181336297
Total Bases9.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA19127741.0548213632045216Illumina PCR Primer Index 6 (96% over 29bp)
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC10277810.5667817293081704No Hit
CCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT5226420.2882169806301934No Hit
TTGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT3153830.17392160599816373No Hit
CGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCTC2691470.14842422860327847No Hit
AAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTCGT2485830.13708397276911416No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCT2330580.12852253181281187No Hit
ATGAGGTAAAGCGAATGATTAGAGGCCTGTAGGCACCATCAATATCTCGT1944080.10720854192803993No Hit
AAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTCG1909380.10529497026180037No Hit
CGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCTC1894010.10444737382058705No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTAT1838360.10137849015412508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)