FastQCFastQC Report
Sat 30 Sept 2023
SRR9641393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80192445
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC42227855.265814005296883No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA25088513.1285378566522066No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC21076942.628294972175995No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA6885180.8585821270320414No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG6295480.785046521527059No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA6065500.7563680094801948No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA6015360.7501155501618638Illumina Single End Adapter 1 (95% over 22bp)
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA5075200.6328775734422363No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA5074690.6328139764288269No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA4477290.5583181807213884No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA4410760.550021887972115No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG3539220.44134082705671335No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC3425840.427202338075613No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC3027400.3775168595994298No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC2983780.37207744445252916No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2928420.36517405099694866No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2834150.35341857951830746No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC2774540.3459852109509817No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA2714960.33855558338444475No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2649260.3303627916569946No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT2576980.32134947375653655No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC2453190.305912857501726No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2394720.29862164696437427No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG2290030.28556680121176004No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC2154820.26870611065668343No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2136110.2663729731647414No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA2087520.2603137988871645No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1966460.24521761370413386No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC1919310.23933800746441888No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG1902500.2372418000224335No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1785690.22267558995114814No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC1760320.2195119502840947No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1742870.21733593482528185No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC1733870.21621363458864487No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG1661490.20718784668555748No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1619290.20192550557599284No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCAC1556880.1941429769350467No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA1527510.19048053716282126No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT1474990.18393129178191286No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA1462780.18240870446087534No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA1372970.17120939509950095No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT1351230.16849841652789113No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1255060.15650601499929326No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1254620.15645114698772433No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG1214910.15149930894362929No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1192170.14866363034572647No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC1189080.14827830726448107No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG1168830.14575313173204782No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC1131570.14110680875237064No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTGTA1096020.13667372281765444No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1088490.13573473161966815No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT1051180.13108217363867633No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCAC1026870.12805071599949347No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACC1020410.1272451538296407No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC1018820.1270468807878348No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA992600.12377724609843235No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA992270.12373609508975565No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG933430.11639874554267549No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG910200.11350196393188909No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA903170.1126253227470493No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAGGC887750.11070244834161123No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA887440.11066379133346041No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA875520.10917736702004784No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC875430.10916614401768147No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAG858900.10710485258305817No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACC858680.10707741857727371No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA849650.10595137733984791No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC829010.10337756879716037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)