FastQCFastQC Report
Sat 30 Sept 2023
SRR9641394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40541937
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC17920464.42022787416398No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA14244053.5134113103673363No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC8224002.0285167923772365No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA4322691.066226806084771Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA3125690.7709769762604091No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA2943520.7260432573806229No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG2900060.7153234933002831No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2789860.6881417629355006No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA2434350.6004523168194948No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA2434220.6004202512573585No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2071610.5109795321323696No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2069160.5103752196151852No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC1672410.4125135905568597No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1667500.4113024989407882No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1632060.40256093338608856No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1267760.3127033619533275No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1263300.3116032665138817No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT1177570.2904572615758344No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1156590.285282373163374No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1100010.2713264538889693No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA1087530.2682481599238833No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1076230.26546092259972676No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC970990.23950261675952977No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA923580.22780855290658658No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC875420.2159294954259339No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA870250.21465427268558976No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC820310.20233616366183982No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC772730.19060016791994916No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG764900.188668834446662No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC759280.18728261552969214No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA758590.18711242139219939No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC742660.18318315673964963No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC736090.18156261256091438No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA723470.1784497864519892No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC714060.17612873306966068No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC707150.1744243251130305No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA693520.1710623742521232No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA684490.1688350509745008No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA679620.16763382568524043No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG671190.16555449730978566No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA666430.16438040441925605No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA653200.16111711682646046No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT651990.16081866044042248No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA589120.1453112612749608No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT587530.14491907478421664No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG586160.14458115309093397No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC583500.14392504235799095No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC578130.14260048798359093No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG576670.1422403670549831No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCAC571860.1410539412559395No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG551340.13599251560180758No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG535490.13208298360288015No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC527470.1301047850772399No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG517180.12756667250506556No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC511920.12626925052939628No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG501940.12380760199000851No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCA492770.12154574656854703No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC488950.12060351235807998No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT484280.11945161870287549No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC481720.1188201737869604No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG480010.11839838831578274No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT479610.118299725047671No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC469460.11579614461933578No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC466270.1150093050561447No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG453990.1119803427251145No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG451440.11135136439090218No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG444840.10972342046705859No Hit
TCGTGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC439230.10833966813179154No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA431970.10654892981556358No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT421500.10396641877273895No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAG412850.10183282559982272No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA407610.10054033678755903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)