FastQCFastQC Report
Sat 30 Sept 2023
SRR9641396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64412013
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC24051533.7340130947312575No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC19931053.09430633692507No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA14956682.322032692876715No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA6431110.99843332019448No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA5709950.886472838537122Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA5342910.8294896792000586No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC3563660.5532601504008267No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA3256280.5055392384647256No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3002650.4661630432199037No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA2771230.43023496253719007No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2721120.4224553578227714No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG2668480.4142829692343259No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC2526100.39217839690866363No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2516920.3907531969230647No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA2204990.3423258950158878No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2090470.3245466028208123No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2058840.31963602814276276No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG2046510.31772178894641906No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2023360.3141277388738029No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1792590.2783005710441001No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC1732250.2689327532738342No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1706420.2649226317457273No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1698750.2637318600801996No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA1671580.2595137028243474No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA1594730.24758269858760665No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1531130.23770876404685565No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1412040.21921997686984257No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG1379740.2142053843279203No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1259150.19548372133626687No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC1180110.18321271841015122No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1169930.1816322678814587No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1158910.17992140689656758No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC1115680.1732099259186326No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC1105690.17165897299312785No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1092420.1695987982862762No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1090720.16933487236301714No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1090420.16928829720008906No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC1065220.16537598351413113No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT1011760.15707628948034896No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA1007180.1563652419929804No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC996810.1547552938611001No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA988370.15344497927739037No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCAC955820.14839157409969472No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCAC946570.14695550657607923No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC932040.14469971618492966No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC921710.14309597807477312No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG912930.14173287830641157No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA896690.13921160948657202No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC854720.13269574419293495No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC853910.13256999125302915No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG825590.12817329587261928No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGC812350.12611777868206045No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG807070.1252980558145264No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA806260.1251723028746206No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT805240.12501394732066518No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC804820.12494874209256587No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA793720.12322546106422726No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAG793210.12314628328724954No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT769410.1194513203616226No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC759760.11795315262076968No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA750690.116545030194911No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT749590.11637425459750808No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT737070.11443051779797658No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT733280.11384211823965197No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA731100.113503672055708No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG722450.11216075485794863No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC719740.11174002588616506No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC713270.11073555487234968No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG712860.1106719021496813No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC701480.1089051509692765No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC687750.10677356101260178No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC679490.10549119152664893No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG677680.10521018804364955No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGG648850.10073431488626197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA644390.10004189746406467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)