FastQCFastQC Report
Sat 30 Sept 2023
SRR9641397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20797022
Total Bases873.4 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA9391024.515559968153133Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3996691.921760721318658No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3550281.707109796777635No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1594320.7666097578778347No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1114340.5358170991981448No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1027590.4941043962928923No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC883540.4248396717568506No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA836310.40212968952958744No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA813540.39118100658834715No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG780040.3750729311148491No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC762540.3666582648227232No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG679760.32685448907059866No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG667470.32094498914315717No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT660740.31770894890624246No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG629530.3027019926218283No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC578720.27827061009023313No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC577810.2778330474430426No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG576760.27732816746551503No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC571700.2748951268119061No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA543540.26135472665269094No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG529670.2546855025685889No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA495670.23833700805817293No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC489760.23549525504180358No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG489620.23542793771146658No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA473160.22751334301612988Illumina Single End Adapter 1 (95% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC463070.22266168685112706No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC455970.21924773652689314No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC452680.21766577926397346No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG450930.21682431263476087No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC438750.21096770489544128No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA435390.20935208896735313No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA435340.20932804706366134No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC412550.1983697473609443No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC405960.19520102445436657No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG388820.1869594598688216No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT375740.1806700978630498No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA371850.1787996377558287No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG362890.17449132861426025No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC356080.1712168213314387No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT349960.16827409231956383No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC349940.16826447555808713No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA347610.16714412284604976No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC347030.16686523676322504No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC340830.1638840407054433No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG334800.16098458712021366No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG326090.15679648749710418No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC325170.15635411646917524No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA323140.15537801517928865No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG316400.1521371665616356No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA306570.14741052829583004No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA297300.1429531593513725No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCAT293490.14112116629005828No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC288620.1387794848704781No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC283220.13618295927176496No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC277260.13331716435170382No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGT276820.13310559559921606No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC275010.13223527868557336No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCAT254480.12236367302972512No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG254400.12232520598381826No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA248900.11968059657772157No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC235170.11307868982395654No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT232930.1120016125385644No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG229290.11025136194980224No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGC226490.10890501534306209No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA224810.10809720737901801No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG224130.10777023748880969No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC223530.10748173464450823No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCA221410.10646235792797641No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA221230.106375807074686No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA220710.10612577127629139No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC217980.10481308333471974No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACC216170.10394276642107703No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC213340.1025819946721218No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACC211730.10180784537324623No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)