FastQCFastQC Report
Sat 30 Sept 2023
SRR9641398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81183086
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC36358804.478617627322026No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA25228103.1075561724766168Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC22033612.714064109363864No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA20246372.4939148038792216No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA5560260.684903749532261No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA5403420.6655844543776028No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA4521340.5569312799959342No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA4340780.5346901939647872No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA4325910.5328585316404454No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA4023860.4956525057448543No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG3921540.48304889518489114No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA3734470.460005917981487No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA3661570.4510262149926156No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC3018890.37186194178427756No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2989040.3681850675151718No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC2817290.34702918290147283No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC2813040.34650567483970734No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG2567050.31620502822472163No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2467620.3039574031467589No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG2380570.29323472625812724No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2290200.28210309718948107No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA2256720.2779790854464439No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2168660.2671319984066632No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG2143810.26407101597492855No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC2101080.25880760433275474No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1849760.2278504170191313No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC1836920.2262688067807622No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1829030.2252969294613905No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1823260.22458619028106422No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1771120.21816367020095787No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA1735490.21377482496785105No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1693410.20859147926453547No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA1624030.20004536412917343No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1620110.19956250492867444No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT1610300.19835412514375225No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1571630.19359081767352376No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA1508920.1858663022492148No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG1488610.18336454960581322No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC1485750.1830122594748369No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC1460260.17987244288791882No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC1455780.17932060380163425No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG1449760.17857907002943937No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT1413900.17416189377181351No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1366750.16835403374540356No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC1354110.1667970591805293No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC1345410.1657254073835035No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA1327060.16346508434035137Illumina Single End Adapter 1 (95% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCAC1302250.16040902904331575No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA1286380.15845418835149971No Hit
CTGTCGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1281630.15786909110599714Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA1273620.15688243238252855No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG1269610.1563884871289569No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG1268930.1563047258390744No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG1238270.15252807709231453No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCAC1179560.1452962751379025No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG1160060.142894296972155No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA1148300.14144571937065809No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC1122260.13823815468162912No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG1071720.13201271998948155No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1042460.12840852095718558No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1038580.1279305888913856No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC1036360.12765713291559277No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCAT1032040.12712500236810412No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1008460.12422045646306176No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCA996680.12276941529421534No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC996360.1227299982166236No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACC985200.12135532763561119No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACC982660.12104245458222664No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA937930.11553268620510436Illumina Single End Adapter 1 (95% over 22bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT935790.11526908449870948No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT921890.11355690519081771No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT896960.11048606848968515No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA895620.1103210094772697No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA892230.10990343481153204No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACC871550.10735610617216497No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT855690.1054024972640237No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA846230.10423722990771749No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC822290.10128833979038442No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCAC819200.10090771863488905No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC816360.10055789207126223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)