FastQCFastQC Report
Sat 30 Sept 2023
SRR9641399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67767820
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC26311363.882574354612558No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA21643613.1937887333545625No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA11446161.6890258532737221Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC10400511.5347269544748525No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA8021821.1837211230935274No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC6286700.9276821948824678No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG4751380.7011262867833139No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3892510.57438914222119No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA3132030.4621706880935524No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCA2861700.4222800733445461No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC2860540.42210890065520773No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2765500.40808454514251746No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC2759000.4071253878315696No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA2485890.3668245488788041No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA2395750.3535232504159054No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC2277000.3360001841582037No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC2222180.3279107989603325No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA2212990.3265546980853154No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC2195040.32390594828046704No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG2150830.31738220293938924No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2132700.31470689185516076No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCA1999330.2950264594611425No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG1973680.2912414771494789No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1935050.28554113146918403No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGC1711760.252591864398176No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1674490.24709220393986409No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1623470.23956355686223932No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC1532680.22616634266824578No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC1528100.2254905056706856No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC1491080.22002773587817934No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG1487830.2195481572227054No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA1457050.21500617844870912No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCA1396750.20610814985637724No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCAT1388320.20486419660540947No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC1333680.19680137268691836No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC1309380.19321559997060553No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1276510.1883652152304737No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1268200.18713896949909264No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG1259710.18588616248833148No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTA1236880.18251730688695608No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA1197670.176731374861992No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC1189040.1754579090783797No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA1177340.1737314259186735No Hit
TGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG1145560.16904188448145446No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1137250.1678156387500734No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1115640.16462680959782977No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC1101250.1625033828740544No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAG1101110.16248272410120318No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT1099520.16224809946667904No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC1091460.16105874440110363No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1091350.16104251250814916No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1087840.1605245675602373No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1079120.1592378211369349No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG1036360.15292804165753007No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1034680.15268013638331587No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC1006120.1484657467216741No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACC984980.14534627202114514No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACC979930.14460108057187024No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCA969480.1430590507411925No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC948120.13990711225475455No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT898940.13264998047745966No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC896120.1322338537671715No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT895820.13218958496820468No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT882040.13015617146899516No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA866820.1279102677347449No Hit
GTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCA852560.12580602415718847No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA847270.12502541766874012No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC846750.1249486850838643No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC838830.1237799887911401No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG796730.1175676006694623No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAG762110.11245898126869065No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACC761930.11243241998931057No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGCTGTAGGCACCATC745760.11004633172499868No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA736780.10872121900925838No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA721730.10650040092775596No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC706220.10421170402117111No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA697090.10286445690594741No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGG693670.10235979259772558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)