FastQCFastQC Report
Sat 30 Sept 2023
SRR9641400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72171970
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC26368093.653508418850144No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA18384862.547368458973754No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC14948872.0712847383825053No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA13532961.87509915553088Illumina Single End Adapter 1 (95% over 22bp)
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA7708871.0681252015152143No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA6239840.8645794205146402No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA3991090.5529972370159772No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3641660.5045809335674224No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA3588290.4971860959317031No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA3225320.4468937178796699No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA3139960.43506641151682574No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2917390.40422756923498143No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA2790130.3865946848894384No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG2735960.37908900089605424No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC2724530.37750528356091706No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2556180.35417905316981096No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA2450930.3395958292395233No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC2330540.3229148379904276No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2078410.2879802227928654No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC1991980.2760046594266445No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC1978310.2741105722900456No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1737200.2407028656693173No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1733980.24025670907971614No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC1724170.23889745561885034No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA1690580.2342432941763956No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1668540.2311894770227278No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC1637410.2268761681300926No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC1622540.2248158114569964No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG1612080.2233664953305279No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC1598760.22152090347540743No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC1573020.21795442191753947No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1547950.21448077418421582No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG1456560.2018179634004725No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA1449260.20080649038678033No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA1442670.19989339351551577No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1442100.19981441548567958No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1441390.19971603934325197No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1424570.19738549467334757No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC1398110.1937192513935812No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG1353010.18747028798022278No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACC1314020.18206791362353No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG1305620.18090402686804863No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG1292280.17905566385398652No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA1290090.1787522219498789No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA1289020.1786039649465021No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA1288850.1785804100954983No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA1275920.17678885583973944No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1189900.16487010123182172No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG1154920.16002334424292422No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC1107500.15345292639233765No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1092690.1514008831960663No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1082350.14996819402324754No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCAC1080080.1496536674833734No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC1044630.14474178825934778No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA1032850.14310957564273222No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT1021640.14155634105595288No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1014230.1405296266680818No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC999340.13846649883604395No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC967710.13408391096986821No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG967390.13403957242680226No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCA959230.13290893957862035No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC956020.13246416856848997No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG949690.1315870967634665No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCAT930680.12895311018945443No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCAC904480.12532289197592916No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC901780.12494878551881014No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACC898760.12453034051862516No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA896520.12421997071716347No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA890860.12343573273668434No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG881990.12220672374607482No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCAC873580.12104145141112262No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT852420.11810956525088619No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC848530.11757057483674065No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC824340.1142188580968484No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA798020.11057201292967338Illumina Single End Adapter 1 (95% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC787640.10913378143897139No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACC776750.10762488539525802No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC773930.10723415198448927No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG740290.10257306264468047No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATT727020.10073439868691404No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCAT721960.10003329547468359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)