FastQCFastQC Report
Sat 30 Sept 2023
SRR9641401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57952005
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA5552600.9581376865218036Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA5263870.9083154241169049No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC4377370.7553440126877405No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC3762460.6492372438192604No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3477800.6001172867099249No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3110750.5367803926714874No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT2937470.5068797878520338No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2665160.4598909045511022No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA2513700.4337554843874686No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2435720.42029952199237974No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1968540.33968453723042025No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1829320.31566120965098615No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT1717250.29632279331836753No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA1599920.27607672935561073No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT1542830.26622547399352275No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1531500.26427040790046863No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA1485250.2562896659054333No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACC1478680.25515596915067906No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC1419750.24498720967462642No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGT1412740.2437775880230546No Hit
ACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC1363520.2352843529745002No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCAC1320040.22778159271624857No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA1293880.2232675124872729No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCA1220340.21057770132370743No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1181280.20383764116530567No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC1153870.19910786520673443No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA1138580.19646947504232165No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC1118590.1930200689346296No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGG1080320.18641632847733225No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1073530.18524466927416922No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1029120.17758143139309848No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1017190.17552283134983854No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGC952640.1643843038735243No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCAC951330.1641582547489082No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCA951220.16413927352470378No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC950530.16402020948196702No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC891220.15378587850411735No Hit
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCA869220.14998963366323564No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCA841120.14514079366192764No Hit
AGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCAT839390.14484227077216744No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC837010.14443158610301748No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACC828770.14300971985352362No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGC817830.14112195082810336No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTC817110.14099771008785633No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCA792780.13679940840700852No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCAC787390.1358693284209925No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA774140.13358295368727965No Hit
TACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACC768240.13256486984358867No Hit
GACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACC764470.13191433152312848No Hit
AGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCA751840.12973494187129506No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA731250.12618200181339714No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC726580.12537616256762815No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA725070.12511560212627673No Hit
TAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGACTGTAG723860.12490680866002823No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATC722710.12470836858880033No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCCTGTAGG721970.12458067671687978No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCAC719900.12422348458866954No Hit
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCA719540.12416136421854601No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGC717920.12388182255299018No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTGTAGGC715280.12342627317208439No Hit
TTGAACGCACGTTCGTGTGGAACCTGGCGCTCTGTAGGCACC708090.12218559133545077No Hit
CACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATC702800.12127276700780242No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCA702430.12120892107184211No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC685790.11833757951946616No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTG684280.11807701907811471No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG683920.11801489870799121No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAG681810.11765080431643392No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTG680690.11745754094271632No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC669200.115474865796274No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCAC652420.11257936632218332No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGG635490.1096579833605412No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACC609460.10516633548744345No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGGC607200.1047763576083347No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCAT595620.10277815236936151No Hit
GCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACC595300.10272293426258504No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG594820.10264010710242036No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCT589860.1017842264473852No Hit
TAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGCTGTAGG586760.10124930103798824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)