FastQCFastQC Report
Sat 30 Sept 2023
SRR9641402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89289732
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA24189592.7091121742867363Illumina Single End Adapter 1 (95% over 22bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC12423181.391333552216284No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC7685700.8607596671921918No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC7571190.8479351242761038No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA7358630.82412947549221No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA4718460.5284437408771705No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA4185760.46878402546890835No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA4077010.4566045735247587No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT3900410.4368262635170638No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA3093340.3464384908222146No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA3089460.3460039503758394No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGG2598370.2910043452700698No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA2485940.2784127518716262No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT2460810.27559831851662403No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA2433780.2725710947368506No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC2373820.26585587691090845No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2353580.26358909891229154No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2273300.25459814349089993No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTC2265330.2537055436564643No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAG2070360.23186988622611163No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCA2032930.22767791485811603No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGT1997200.2236763349228106No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1991620.22305140304374527No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC1916900.21468313960221092No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA1906170.21348143367705483No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA1896200.21236484392180727No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1862940.20863989153870457No Hit
ACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC1834420.20544579526792622No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGC1770540.1982915571971926No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG1728840.19362136734826352No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACC1658750.1857716405734088No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC1654150.18525646375554136No Hit
AAAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGT1653890.1852273450658358No Hit
AGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCAT1623260.1817969394286008No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCAC1558010.17448926826211103No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG1509960.169107910414604No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCAC1490730.16695424732599715No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCAC1448290.16220118120636762No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1445510.161889835216439No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1403580.15719388652661653No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGG1376290.1541375440571375No Hit
TTGAACGCACGTTCGTGTGGAACCTGGCGCTCTGTAGGCACC1310190.14673467717430264No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG1284530.14386088649028536No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCA1276880.14300412504317966No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC1272350.14249678787254058No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1206780.1351532783187209No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1188460.13310153064408345No Hit
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCA1186180.13284618213435784No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCT1179480.13209581589963781No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC1156390.12950985226386388No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1155240.12938105805939704No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGC1154670.12931722093196563No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC1130300.12658790374687204No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1115150.12489118009672154No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAG1109890.12430208660498612No Hit
CATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAA1091370.12222793993826749No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC1065570.11933846995979336No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA1059420.11864970095329663No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1047170.11727776268832343No Hit
TGGGCGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAG1046690.11722400510732857No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1036190.11604805802306586No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1019340.1141609429402252No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTG1005420.11260197309137404No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCAC989380.11080557392646223No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCCTGTAGG977110.10943139576228093No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCA976190.10932836039870744No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA964120.10797658122660733No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTGTAGGC960580.10758011906677019No Hit
TACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACC959930.10750732234250632No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT946070.1059550721912795No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACC940080.10528422237844773No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCAC933440.10454057584135207No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA930540.10421579045617473No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC930450.10420571090973821No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA927530.10387868562535275No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCA903280.1011628078355079No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCA894540.1001839718815597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)