FastQCFastQC Report
Sat 30 Sept 2023
SRR9641403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19468659
Total Bases817.6 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3935092.0212434765024136No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3233631.6609413108524835No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA2838471.4579689335562351Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2368161.2163960548078838No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2177231.1183256124625738No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1472480.7563335512733569No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC1227540.6305210851964689No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA914740.469852597449059No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCAT791100.406345398519744No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA653910.33587829546965714No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA647020.33233927411230535No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA601580.3089991971198427No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG561850.28859203913325515No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC520110.2671524525649147No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC503870.25881084054120007No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC466390.23955938619090303No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGC446680.22943542233699815No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG445180.22866495324613778No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG428670.22018465678606833No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG425820.21872076551343367No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC422230.21687677615597456No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT415610.21347643923497764No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG410420.21081061618060085No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC384450.19747122798750547No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT378190.19425580364831496No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC377330.1938140680362217No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA373440.19181598486059057No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC361780.18582687179430282No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC352860.18124514893398666No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG347990.17874369261899342No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG342690.17602136849795355No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT319270.16399177775932075No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC317380.16302098670483675No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA306040.15719624037793256No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC300940.15457664546900737No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC299710.1539448608145019No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC291740.14985110171173063No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC291550.14975350896022166No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA287670.14776056224519624No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG281370.1445245920635828No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC279110.14336375196668655No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC273940.14070820183352126No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA271120.1392597199427038No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC265780.136516849979241No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC260960.13404107596727644No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC254700.13082565162808596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC246310.12651616117987377No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA241160.12387088396791993No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG241130.12385547458610273No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT240730.1236500161618733No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGT237380.12192930185895186No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG233370.1198695811560519No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG232610.11947921015001599No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA230000.11813859393191897No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG226640.11641274316839183No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA225820.1159915533987215No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA225480.1158169137381265No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC219840.11291994995649161No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA218570.11226761945956319No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG217060.11149201390809711No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA216200.11105027829600386No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACC214800.11033117381120086No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA212860.10933470045368816No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC209200.10745475587198892No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG206190.1059086812296625No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC200030.10274462149652937No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC198920.10217447436929271No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC196220.10078763000574412No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCAC195150.10023802872093038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)