FastQCFastQC Report
Sun 1 Oct 2023
SRR9641404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22712238
Total Bases953.9 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA9408524.142489172577356Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA3928541.7297018462029148No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3312581.4585000386135438No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC2703561.1903538524032726No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2063170.9083957292099529No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC1360710.5991087271980858No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1333650.5871944455671871No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1154340.5082458188400456No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1138840.5014213042325464No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT914890.40281807543580694No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA727080.3201269729561657No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA645030.2840010746629196No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA564130.24838151132442343No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT549890.2421117637108241No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC543200.23916621514797443No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC538530.2371100549404246No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG505330.2224923849424262No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC501140.2206475645420764No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC493350.21721769558772674No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA450050.1981530838132288No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG449850.1980650255602288No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC442170.19468358864502916No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC431250.1898756080312297No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC427510.18822891870012987No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG421420.18554754489628014No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC421210.18545508373063016No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT406600.17902242834898086No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA400600.17638068075898114No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA392380.17276148656068152No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC382920.16859633119378195No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA382900.16858752536848196Illumina Single End Adapter 1 (95% over 22bp)
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC373650.1645148311672324No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC365870.16108936512553276No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC344060.15148661263588378No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC343640.1513016903045838No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC337820.1487391951422841No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT335940.14791144756408417No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG322370.1419366950980348No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC319770.14079193780903493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA317630.13984971450193504No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA315760.1390263698363851No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG309910.13645066593613542No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC307400.13534553486098552No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC304130.13390578242443565No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC300810.13244401542463585No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC300310.13222386979213585No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC299060.13167350571088593No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC296830.130691656189936No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA291470.12833169500953626No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC291110.12817319015413628No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC290240.12779013675358633No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA284010.1250471221726366No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA273940.1206133891340871No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG265000.1166771852249875No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG262520.11558526288778764No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG259080.1140706609361878No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCA252760.1112880201413881No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA252570.11120436480103811No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA235690.10377224824783889No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATT232940.10256144726908903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)