FastQCFastQC Report
Sat 30 Sept 2023
SRR9641405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22545064
Total Bases946.8 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA11752605.212937075716441Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA4583772.0331590098834935No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3735621.6569569285764723No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA3382921.5005147024643621No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2294451.0177172262629195No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1661330.7368930068240214No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1434590.6363211033687907No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG983220.43611319976736374No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA914570.40566307551843717No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT878560.3896906214149581No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC825560.3661821496714314No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG795480.3528399830668035No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA648610.28769490297299666No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG631440.2800790452402353No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT608010.269686526505314No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC590770.2620396198476083No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG588490.26102831200656607No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC554890.2461248280333114No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC548890.24346349160951597No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC539590.23933842015263296No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC538350.23878841062504857No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC538270.23875292613939797No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC520840.23102174382827212No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA502820.22302886343547307No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG502240.22277160091450618No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT495130.2196179172523085No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC484760.21501824079984871No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA475050.21071131135400634No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA461160.20455031753291983Illumina Single End Adapter 1 (95% over 22bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC460230.20413781038723156No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG439400.19489853743595492No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC433400.19223720101215946No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT429810.1906448347185885No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC418160.18547740649571898No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG414470.18384068459508476No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA406040.18010150691965213No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA400730.17774622418459313No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG400140.1774845261029199No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC398870.17692120989321655No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC395840.17557723499919983No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA379940.16852469347614182No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC367560.1630334693217105No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG366910.16274515787579935No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG365850.1622749884409288No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC364490.16167175218486848No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGT357480.15856242412973412No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG356270.1580257212842687No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA353090.1566152129796571No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG350890.1556393896242654No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC350510.15547083831742506No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC343930.15255223937266268No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA342430.1518869052667138No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG340140.15087116186496521No Hit
TGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG324160.1437831358562566No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGG318600.14131696410353947No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA318130.14110849275034218No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG318130.14110849275034218No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC307130.1362293759733838No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG302890.13434869823390166No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA302460.13415796912352965No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA293700.13027241794478828No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC292660.1298111196313304No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC290990.12907038099337398No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG289830.12855585595144017No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC284980.12640460900887218No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA283510.1257525815850423No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC282990.12552193242831333No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC278350.12346383226057818No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA276900.12282067595816096No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC269990.11975570351008985No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC269390.11948956986771028No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG267900.11882867132246774No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACC259600.11514715593621734No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAG249180.11052530168022588No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA230270.10213765638456382No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCTGTAGGCA229200.10166305138898696No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACC227340.10083803709761037No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC225730.10012391182389192No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)