FastQCFastQC Report
Sat 30 Sept 2023
SRR9641406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20038055
Total Bases841.5 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA6397183.1925154412441725Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA5033242.511840595307279No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC4244632.1182844342926495No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC3482881.7381327678759242No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2054091.02509450143739No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1296670.6471037233903191No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC1251480.6245516343776879No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1212370.6050337719903454No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1184980.5913647806635923No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1007080.5025837088479895No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA809660.4040611726038281No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA786050.3922785919092447No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG711110.35487975255083387No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC704770.35171577281327954No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT692480.34558244300656926No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA687110.34290254218785204No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG607870.3033577859727404No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC576260.2875828018238297No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC575060.2869839413056806No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG567330.2831262814679369No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC555100.27702289468713404No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC535480.2672315252153964No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA483700.2413906938572631No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA481200.24014306777778582No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC480430.23975879894530683No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT469750.23442894033377992No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGC464670.23189376414028207No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA457930.22853016423001132No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCA440170.2196670285614048No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC435670.2174213016183457No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC418860.2090322638599405No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC410090.2046555915731342No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC405110.2021703204228155No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA404550.20189085218101258No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC401490.2003637578597324No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA397060.19815296444689864No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC395450.1973494932517153No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA374450.1868694341841062No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG370140.18471852682308737No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC367450.18337608116156984No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG357170.17824584272275928No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA353620.17647421368990154No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAG347840.1735897021941501No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA341000.17017619724070027No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC328230.16380332322673033No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC319610.1595015085046927No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGG314130.1567667121384785No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACC308230.15382231459091214No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG305000.1522103816962275No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA302360.1508928885562995No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCA301720.1505734962799533No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA297370.14840262690166287No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC293290.14636650113995595No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA292700.14607206138519932No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG290050.1447495777409534No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC288950.1442006222659834No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAG287590.14352191367874775No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG283600.14153070245590205No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA278210.13884082062854902No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC277830.13865118146446848No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG274360.13691947646615402No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGG271730.13560697383054393No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA266690.13309175965431774No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGT264760.13212859232096127No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGG262310.13090591876307356No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC260760.13013239059379766No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA258160.1288348594711413No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCAC250480.1250021521549871No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC245590.12256179554352956No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCAT244450.1219928780512879No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG242200.12087001457975836No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACC236390.11797053157105318No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC233550.116553228344767No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCACTGTAG232740.11614899749501635No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC230100.11483150435508836No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG217660.10862331698360944No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT207130.10336831593685115No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA206740.1031736862684527No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC206700.10315372425118106No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT203680.10164659194717252No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC201320.10046883292814596No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCAT201090.10035405132883407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)