FastQCFastQC Report
Sat 30 Sept 2023
SRR9641407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49937018
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA18226953.64998767046923Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA8132071.6284652800053059No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC7222951.4464119583592276No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA6143031.2301555531409585No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC6083121.2181584410987456No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC5879241.1773310132375143No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC4802060.9616232991725697No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2989450.5986440760239228No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT2218850.4443296954575862No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT1890750.37862693363067856No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1678750.33617345753404815No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC1596250.31965264726059534No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC1483920.2971583124967534No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1407750.2819050989388273No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1392390.278829224444279No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT1352550.2708511749740443No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1295310.2593887364279541No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1262350.25278842240840255No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1241130.2485390697538247No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA1238320.2479763609432986No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA1215340.24337456433622048No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC1191480.2385965457528922No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC1183520.23700253787681114No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1095080.2192922292636697No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT1084270.217127502487233No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1034990.20725907181722383No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA997060.1996635041363503No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC970410.1943267817874107No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA915480.18332692592897717No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC913770.18298449458876379No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC898120.17985054694295122No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCAT889770.17817844069103206No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC821950.1645973333850251No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC806490.16150143366590292No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA801510.1605041774821236No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC789830.15816523125189413No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC785730.15734419704436495No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG778390.155874345560642No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC772150.15462477154723175No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA765110.15321499573723044No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC744600.1491078221771272No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC742540.1486953025509052No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG726100.14540315563095899No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG699560.14008846102905062No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACC698380.13985216337907883No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC698060.1397880826604424No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAG671360.13444134769921584No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA661190.13240478236005201No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA641470.12845580807408244No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGG629870.1261328820235121No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC628550.12586854905913686No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA627360.12563024888670765No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCA626990.12555615555578428No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCA618050.12376590047887921No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC613150.12278466447475898No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCAC607670.12168728216811024No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATT604860.12112457335758414No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA597760.11970278241283852Illumina Single End Adapter 1 (95% over 22bp)
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG588700.11788849706644478No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA584490.11704543511188434No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACC576620.11546944993791981No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACC567870.11371724278770509No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC561600.11246166120692268No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA555120.11116402665453512No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC554570.11105388791937877No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGT551560.11045112865970491No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCA546580.10945387247592558No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC543160.10876900979549879No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC541690.10847463899426274No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA538670.1078698772121315No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA535770.1072891456994889No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA528240.10578124628907556No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG525410.10521453243363471No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA522090.10454969497778181No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGC519870.10410513499224162No Hit
AGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCAT515900.1033101335766585No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT505140.10115541941250877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)