FastQCFastQC Report
Sat 30 Sept 2023
SRR9641408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57352017
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA18710893.2624641605891562Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC8604531.500301201263767No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA8591441.4980188055112342No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA7577181.3211706224734867No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC6814111.1881203759581813No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC5892291.027390196233203No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC5797621.0108833661421184No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA3477750.6063866942988247No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT2716600.4736712224087951No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT2488210.43384873456150636No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1900090.3313030821566398No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC1792390.312524318020062No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT1762490.3073109006785236No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC1719040.2997348811638133No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1640270.2860004034382958No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1631030.28438930055415484No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA1582020.275843829520416No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC1571280.27397118396027814No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1491280.2600222412404432No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA1482360.25846693412718164No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC1378270.24031761603083637No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC1344920.2345026505345052No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1335310.23282703379028502No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCA1313750.22906779372728947No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATC1312290.22881322552265249No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA1282590.22363468053791377No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1249350.21783889483782234No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT1245080.21709437002015117No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1232510.21490264239529708No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCA1227440.21401862815042758No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1199130.20908244604544596No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACC1018800.17763978553709803No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC1006060.1754184164089643No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATC988930.17243159904907965No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC941640.16418603028381723No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGG898400.15664662674374644No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCA876680.15285948879531125No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA863470.1505561696286985No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC858530.1496948224157487No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCAT858050.14961112875942967No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC853540.148824757113599No Hit
AGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGC823590.14360262168286078No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAG822240.14336723327446357No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA818970.1427970702407903No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA812990.14175438677248264No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC811950.1415730505171248No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC811250.14145099726832625No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCAC803840.14015897644890152No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC798030.13914593448387352No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA797820.13910931850923394No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG785740.13700302815853888No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA778820.13579644461327317No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA768040.1339168245817754No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC763310.13309209334346514No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTA738080.1286929455331972No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATT724760.12637044657034469No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCA720620.12564858878459323No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG706740.12322844722270188No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA700420.1221264807478349No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA691000.12048399274257433No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCAC687680.11990511161970119No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA683660.11920417724802948No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGT682010.11891648030443289No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAG674950.11768548610940745No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG664890.1159314065623882No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCCTGTAGGCACC664670.11589304696990865No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATC659480.11498810931095937No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT619640.10804153583648156No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCA619340.10798922730128219No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGC618220.10779394210320449No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC614970.1072272663052112No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGC606430.10573821666986882No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT596460.10399982968340939No Hit
GCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC596150.10394577753037003No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACC589390.10276709187054398No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTC581850.10145240401919953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)