FastQCFastQC Report
Sat 30 Sept 2023
SRR9641409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9641409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43320304
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA14700433.393427248340639Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC10798302.492664871419185No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA7332441.6926104673688345No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC6926711.5989523065212101No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC3285900.7585126826441476No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG2424090.55957363549434No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACC2182420.5037868616988468No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA2100310.48483270108169135No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1849270.42688296924232105No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCC1654200.38185327600655805No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA1594490.3680699008945089No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA1539700.3554222518844743No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1267630.2926179834749082No Hit
CTGGAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1248860.28828514222799545Illumina Single End Adapter 1 (95% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCA1155030.26662555276620403No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC1129450.2607207003902835No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCA1088660.25130479232094033No Hit
CTGTCGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1076170.2484216177245663Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA1051440.24271297819147344Illumina Single End Adapter 1 (95% over 22bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC1024050.23639030787964924No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA992660.22914428301334172No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT937000.21629580438770699No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA933610.2155132614027824No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAG922830.21302482087844996No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCA900040.20776400830428152No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC869830.20079037303154657No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCA838830.19363437523430121No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTC819240.18911224630371937No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGC806580.18618982913877982No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA761710.1758320994238637No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTC743030.1715200336544268No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG720890.16640926619536187No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGT690830.15947025671842008No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTA673270.1554167302242385No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC666680.1538955035957273No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC658580.15202571062289866No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCAT629550.1453244649437363No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATC627880.14493896441723955No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA626670.14465964966450837No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGC606190.139932074345554No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCAC601290.13880096501631198No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAG589130.13599396717068282No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCA577860.1333924157134262No Hit
CTTTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA562600.12986981808807252Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG556520.12846631916525794No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCA551010.12719439826645723No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT539510.12453975392231781No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCAC534970.1234917465029793No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCAT523420.12082556022690884No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG521700.12042851776848103No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA520510.12015381978852226No Hit
AAAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGT513980.11864644347832831No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACC509570.11762844508201051No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACC506610.11694516271169289No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC499590.11532467546857474No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA491790.11352413408733235Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA474960.10963911979934397No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCA472690.10911511608967471No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGG471150.10875962458619864No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCA466260.10763082364334287No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT463620.1070214096373839No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTA462310.10671901102079062No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG461920.10662898395172851No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCAC452670.10449372654448594No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCAT439330.10141433910528422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)