FastQCFastQC Report
Thu 28 Sept 2023
SRR9651902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75958752
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT20842082.743868145700972RNA PCR Primer, Index 8 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC8819341.1610696289480902RNA PCR Primer, Index 23 (95% over 22bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT8002401.0535191520787492Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT7740081.0189846194418781RNA PCR Primer, Index 3 (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG6993360.9206786335826054Illumina Paired End PCR Primer 2 (100% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGG4255670.5602606530449579Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTG4153610.5468244133342265Illumina PCR Primer Index 6 (95% over 23bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4108190.5408448522166346TruSeq Adapter, Index 2 (96% over 27bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT4103380.5402116137979729Illumina PCR Primer Index 9 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT2938520.38685733014676177RNA PCR Primer, Index 3 (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG2700790.3555600808186001Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA2686870.35372750726604885RNA PCR Primer, Index 2 (96% over 27bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT2493760.3283044987363668RNA PCR Primer, Index 30 (95% over 23bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2317270.30506951983623953Illumina PCR Primer Index 5 (95% over 21bp)
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG2155070.28371582513625293RNA PCR Primer, Index 13 (96% over 31bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC1911250.25161682487885No Hit
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1875480.24690769011054842RNA PCR Primer, Index 23 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1838130.24199054771200032Illumina Paired End PCR Primer 2 (100% over 24bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT1613760.2124521477130114RNA PCR Primer, Index 24 (96% over 28bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT1540200.20276794437065002TruSeq Adapter, Index 10 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG1497090.19709249567449447RNA PCR Primer, Index 35 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1482910.1952256930182318Illumina PCR Primer Index 12 (95% over 24bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCG1415690.1863761532048341RNA PCR Primer, Index 30 (95% over 22bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT1355660.1784731797594568Illumina PCR Primer Index 2 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG1307410.17212104801300582RNA PCR Primer, Index 15 (96% over 29bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1273510.1676580994906288RNA PCR Primer, Index 35 (96% over 26bp)
GACGCCTTATTCGTATCCATCTATATTTATCTCGTATGCCGTCTTCTGCT1245060.1639126456422033Illumina PCR Primer Index 2 (96% over 26bp)
TGCGATTCGAAAAGTTATTATGAATCATCAAATCTCGTATGCCGTCTTCT1078150.1419388775634439Illumina PCR Primer Index 9 (95% over 24bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT1046350.1377523948787363Illumina PCR Primer Index 5 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATATCTCGTATGCCGTCTTCTGCTTG1031040.13573682727172767Illumina PCR Primer Index 2 (96% over 28bp)
GACGCCTTATTCGTATCCATCTATATTAATCTCGTATGCCGTCTTCTGCT979580.1289620977448392Illumina PCR Primer Index 8 (96% over 25bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT897230.11812068739623315RNA PCR Primer, Index 13 (96% over 31bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT861990.11348132733934332TruSeq Adapter, Index 1 (96% over 29bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG836540.11013082468758834No Hit
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT805360.10602596525019262RNA PCR Primer, Index 1 (95% over 22bp)
GCGAGAATCATACCACTAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA769640.10132341300183552RNA PCR Primer, Index 39 (96% over 30bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC768170.10112988691546695RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)