FastQCFastQC Report
Thu 28 Sept 2023
SRR9651903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73414105
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT16354852.227753100034387RNA PCR Primer, Index 8 (96% over 27bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT8210511.1183831771837305Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT6880210.9371782166383421RNA PCR Primer, Index 3 (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG5667450.7719838033849218Illumina Paired End PCR Primer 2 (100% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC5603760.7633083587956293RNA PCR Primer, Index 23 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG5303140.7223598244506284Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTG4044200.5508750668553407Illumina PCR Primer Index 6 (95% over 23bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4029110.5488196035353152TruSeq Adapter, Index 2 (96% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGG3561880.4851765202340885Illumina Paired End PCR Primer 2 (100% over 24bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT3275850.44621534240593136Illumina PCR Primer Index 9 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT2620210.35690825352975425RNA PCR Primer, Index 3 (95% over 21bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA2532070.34490238626487374RNA PCR Primer, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2018010.27488041977764355Illumina PCR Primer Index 5 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC1990350.27111275142562863No Hit
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG1883440.2565501547693049RNA PCR Primer, Index 13 (96% over 31bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT1720210.23431600780258777RNA PCR Primer, Index 30 (95% over 23bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1599080.21781645366377483RNA PCR Primer, Index 23 (96% over 29bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT1531120.20855937697531013TruSeq Adapter, Index 10 (96% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1477200.2012147393201892Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG1422950.19382515117496835RNA PCR Primer, Index 35 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1376610.18751301265608292Illumina PCR Primer Index 12 (95% over 24bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT1331710.18139702173036093RNA PCR Primer, Index 24 (96% over 28bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT1200690.16355031502461279Illumina PCR Primer Index 2 (96% over 28bp)
GACGCCTTATTCGTATCCATCTATATTTATCTCGTATGCCGTCTTCTGCT1183590.1612210623557966Illumina PCR Primer Index 2 (96% over 26bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1167630.15904709319823485RNA PCR Primer, Index 35 (96% over 26bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG1124800.15321306443768537RNA PCR Primer, Index 15 (96% over 29bp)
TGCGATTCGAAAAGTTATTATGAATCATCAAATCTCGTATGCCGTCTTCT1093620.1489659241912709Illumina PCR Primer Index 9 (95% over 24bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT994230.13542765385480077Illumina PCR Primer Index 5 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATATCTCGTATGCCGTCTTCTGCTTG979350.13340079539211164Illumina PCR Primer Index 2 (96% over 28bp)
GACGCCTTATTCGTATCCATCTATATTAATCTCGTATGCCGTCTTCTGCT927380.1263217742693996Illumina PCR Primer Index 8 (96% over 25bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCG841330.11460059344181885RNA PCR Primer, Index 30 (95% over 22bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATATCGTATGCCGTCTTCTG802210.10927191716087802TruSeq Adapter, Index 6 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT764000.10406719526172797RNA PCR Primer, Index 13 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)