FastQCFastQC Report
Thu 28 Sept 2023
SRR9651904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65054407
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT17139992.6347161999340027RNA PCR Primer, Index 8 (96% over 27bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT7646761.1754407353217438Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT6500890.999300477829273RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC6461860.9933008842890537RNA PCR Primer, Index 23 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG5272900.8105369402567916Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTG4791360.7365158212878644Illumina PCR Primer Index 6 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG3701920.5690498416194925Illumina Paired End PCR Primer 2 (100% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGG3408970.5240183036331421Illumina Paired End PCR Primer 2 (100% over 24bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT3393470.5216356825756632Illumina PCR Primer Index 9 (96% over 27bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG3339910.5134025739409169TruSeq Adapter, Index 2 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT2469790.37964991364843276RNA PCR Primer, Index 3 (95% over 21bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT2312130.3554148145566833RNA PCR Primer, Index 30 (95% over 23bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA2243420.3448528859851109RNA PCR Primer, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT1900900.2922015721394555Illumina PCR Primer Index 5 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC1630640.25065788394627897No Hit
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG1618060.24872411795253163RNA PCR Primer, Index 13 (96% over 31bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1577170.24243860988541485RNA PCR Primer, Index 23 (96% over 29bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT1344110.20661321223018758RNA PCR Primer, Index 24 (96% over 28bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT1290690.1984016240437024TruSeq Adapter, Index 10 (96% over 27bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1248820.19196547283875787Illumina PCR Primer Index 12 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1225490.18837924385353325Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG1222260.18788273636865216RNA PCR Primer, Index 35 (96% over 28bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT1129180.1735747126247727Illumina PCR Primer Index 2 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG1084100.16664512828469868RNA PCR Primer, Index 15 (96% over 29bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1063520.16348162239031708RNA PCR Primer, Index 35 (96% over 26bp)
GACGCCTTATTCGTATCCATCTATATTTATCTCGTATGCCGTCTTCTGCT1056750.16244095499940533Illumina PCR Primer Index 2 (96% over 26bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCG1029860.15830749175839848RNA PCR Primer, Index 30 (95% over 22bp)
TGCGATTCGAAAAGTTATTATGAATCATCAAATCTCGTATGCCGTCTTCT897750.13799987447429962Illumina PCR Primer Index 9 (95% over 24bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT879830.13524525709687893Illumina PCR Primer Index 5 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATATCTCGTATGCCGTCTTCTGCTTG872090.1340554837430153Illumina PCR Primer Index 2 (96% over 28bp)
GACGCCTTATTCGTATCCATCTATATTAATCTCGTATGCCGTCTTCTGCT836030.1285124311409064Illumina PCR Primer Index 8 (96% over 25bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT754860.11603518267409615RNA PCR Primer, Index 13 (96% over 31bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT732640.11261958010008452TruSeq Adapter, Index 1 (96% over 29bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT719570.11061049253742333RNA PCR Primer, Index 1 (95% over 22bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG682020.10483840087882133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)