FastQCFastQC Report
Thu 28 Sept 2023
SRR9651905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72822891
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT19523092.680900158165926RNA PCR Primer, Index 8 (96% over 27bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT10989331.5090488511366569Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC10157491.3948210323042518RNA PCR Primer, Index 23 (95% over 22bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTG8907461.2231675888835558Illumina PCR Primer Index 6 (95% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT6603260.9067560912955241RNA PCR Primer, Index 3 (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG5642430.774815435437739Illumina Paired End PCR Primer 2 (100% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGG4757990.6533646130582759Illumina Paired End PCR Primer 2 (100% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG4741360.6510809904539495Illumina Paired End PCR Primer 2 (100% over 24bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4400950.6043360733920876TruSeq Adapter, Index 2 (96% over 27bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT3615050.496416710509337Illumina PCR Primer Index 9 (96% over 27bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT3167170.43491407118127184RNA PCR Primer, Index 30 (95% over 23bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA2813940.38640871865413856RNA PCR Primer, Index 2 (96% over 27bp)
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG2630970.36128337722818504RNA PCR Primer, Index 13 (96% over 31bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT2463490.3382851142232186RNA PCR Primer, Index 3 (95% over 21bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT1996090.27410199905411614Illumina PCR Primer Index 5 (95% over 21bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1580120.21698122366495998RNA PCR Primer, Index 23 (96% over 29bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT1555830.21364573400416087TruSeq Adapter, Index 10 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG1478730.20305840370989942RNA PCR Primer, Index 35 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT1441120.19789381885429402RNA PCR Primer, Index 24 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1328000.1823602416443478Illumina PCR Primer Index 12 (95% over 24bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT1225070.16822594972232013Illumina PCR Primer Index 2 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1181620.16225941922574869RNA PCR Primer, Index 35 (96% over 26bp)
GACGCCTTATTCGTATCCATCTATATTTATCTCGTATGCCGTCTTCTGCT1160030.1592946921044373Illumina PCR Primer Index 2 (96% over 26bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG1146640.1574559845474962RNA PCR Primer, Index 15 (96% over 29bp)
TGCGATTCGAAAAGTTATTATGAATCATCAAATCTCGTATGCCGTCTTCT1095820.15047740963758222Illumina PCR Primer Index 9 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG1059100.14543503910054875Illumina Paired End PCR Primer 2 (100% over 24bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCG1036100.14227669154194936RNA PCR Primer, Index 30 (95% over 22bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGAATCTCGTATGCCGTCTTC1015810.1394904797174284RNA PCR Primer, Index 40 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATATCTCGTATGCCGTCTTCTGCTTG953480.13093135783362406Illumina PCR Primer Index 2 (96% over 28bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT920830.12644787749500358Illumina PCR Primer Index 5 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATTAATCTCGTATGCCGTCTTCTGCT904130.1241546425285423Illumina PCR Primer Index 8 (96% over 25bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC867020.1190587174024717No Hit
CCCCACTACCACAAATTATGCAGTCGAATATCGTATGCCGTCTTCTGCTT840020.11535109200759416RNA PCR Primer, Index 46 (96% over 26bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT760090.1043751476441659RNA PCR Primer, Index 13 (96% over 31bp)
GCGAGAATCATACCACTAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA741490.10182100570547248RNA PCR Primer, Index 39 (96% over 30bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC730030.10024732470453555RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)