FastQCFastQC Report
Thu 28 Sept 2023
SRR9651906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57305183
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT19599743.420238619602698RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT7725531.3481380907552463RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC7526831.313464089277928No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC7317031.2768530902344384RNA PCR Primer, Index 23 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT5655900.9869787868926271RNA PCR Primer, Index 8 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTT4007260.6992840420734718No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTC3917720.6836589283730234No Hit
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG3650930.6371029301136688TruSeq Adapter, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2942590.5134945647063024Illumina PCR Primer Index 5 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTT2942060.5134020774351249No Hit
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA2532330.4419024366434708RNA PCR Primer, Index 2 (96% over 27bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT2513790.43866712719510903TruSeq Adapter, Index 10 (96% over 27bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT2409050.4203895483590027RNA PCR Primer, Index 1 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG2383100.4158611621570077Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG2088250.3644085736538002RNA PCR Primer, Index 35 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1883820.32873466262205286Illumina PCR Primer Index 12 (95% over 24bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATTCCGTCTTCTGCTT1760090.3071432474092265RNA PCR Primer, Index 46 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCTCGTATGCCGTCTTCT1650710.28805596868960354RNA PCR Primer, Index 17 (95% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGGGTATTCCGTCTTT1480680.25838500507013473No Hit
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT1438210.2509738080759641Illumina PCR Primer Index 5 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT1405880.24533208453413366Illumina PCR Primer Index 9 (96% over 27bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTGCT1356640.2367394935288838RNA PCR Primer, Index 31 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATTCCGTCTTC1339270.23370835409425358No Hit
CGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCTGC1336670.23325464295262788RNA PCR Primer, Index 15 (96% over 25bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT1267910.22125572829948034TruSeq Adapter, Index 1 (96% over 29bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT1227290.21416736423300486RNA PCR Primer, Index 30 (95% over 23bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1197390.20894968610430928RNA PCR Primer, Index 35 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTA1139900.19891743474582396No Hit
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1027610.17932234855615067RNA PCR Primer, Index 23 (96% over 29bp)
CCTGGAGGTACTGCAATACCAGGTCAATCTCGTATGCCGTCTTCTGCTTG977570.17059015412270823RNA PCR Primer, Index 15 (96% over 31bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTC965050.1684053604714952Illumina PCR Primer Index 12 (95% over 22bp)
GGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTC924490.1613274666621342No Hit
TCAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTT913460.15940268439592978Illumina PCR Primer Index 12 (96% over 27bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTG884050.15427051336700207RNA PCR Primer, Index 31 (95% over 24bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG879490.15347477382630467RNA PCR Primer, Index 15 (96% over 29bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCT815940.142385026499261RNA PCR Primer, Index 15 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG808780.1411355758169379Illumina Paired End PCR Primer 2 (100% over 24bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT782550.13655832841507545Illumina PCR Primer Index 2 (96% over 28bp)
CCTTCGAGCCGGAAACGAACCAGCGACCTATCTCGTATGCCGTCTTCTGC779100.1359562886309952Illumina Paired End PCR Primer 2 (96% over 26bp)
CGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGATCTCGTATGCCGTCTTC741140.1293321059632599RNA PCR Primer, Index 17 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGGGTATGCCGTCTTT731550.127658609867802No Hit
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCGCGTATGCCGTCTTCT724920.12650164645665646RNA PCR Primer, Index 23 (95% over 21bp)
ACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCT689630.12034339023051371Illumina PCR Primer Index 12 (95% over 23bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTATCTCGTATGCCGTCTT678230.11835404137877023Illumina PCR Primer Index 1 (95% over 21bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG678120.11833484590739377No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATTCCGTCTT677600.11824410367906862No Hit
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC676350.1180259733225178RNA PCR Primer, Index 35 (95% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGGGTTTTCCGTCTTT664890.11602615421365987No Hit
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT642270.11207886728151623RNA PCR Primer, Index 13 (96% over 31bp)
CAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTTG639940.11167227229690549Illumina PCR Primer Index 12 (96% over 28bp)
CCTGGAGGTACTGCAATACCAGGTCAAATCTCGTATGCCGTCTTCTGCTT635150.11083639677060275RNA PCR Primer, Index 13 (100% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTA627300.10946653813146359No Hit
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTT606300.10580194814140984TruSeq Adapter, Index 2 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)