FastQCFastQC Report
Thu 28 Sept 2023
SRR9651907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55484107
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT19112603.444698136711473RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC16724493.01428479330126RNA PCR Primer, Index 23 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT7750651.3969135341765526RNA PCR Primer, Index 8 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC7514981.3544383078923843No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT7309131.317337593628388RNA PCR Primer, Index 3 (95% over 21bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4860930.8760941218716921TruSeq Adapter, Index 2 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA3339220.601833602548564RNA PCR Primer, Index 2 (96% over 27bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT2906190.5237878299095631TruSeq Adapter, Index 10 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2795400.5038199497380393Illumina PCR Primer Index 5 (95% over 21bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG2446320.4409046359888247RNA PCR Primer, Index 35 (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTC2129290.3837657511546505No Hit
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT2118620.3818426779401893RNA PCR Primer, Index 1 (95% over 22bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT1891920.3409841308250667RNA PCR Primer, Index 30 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG1829660.32976289949120025Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1786380.32196246755850283Illumina PCR Primer Index 12 (95% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTT1741670.3139043041640735No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATTCCGTCTTC1703220.30697439178393915No Hit
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT1588970.28638290961409907Illumina PCR Primer Index 9 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCTCGTATGCCGTCTTCT1585620.285779133112839RNA PCR Primer, Index 17 (95% over 23bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTGCT1389940.25051137616759334RNA PCR Primer, Index 31 (96% over 26bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT1373200.24749429597920716Illumina PCR Primer Index 5 (95% over 22bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1338650.2412672875856144RNA PCR Primer, Index 35 (96% over 26bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT1320580.23801049911463837Illumina PCR Primer Index 10 (96% over 26bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCTGC1231420.22194103259154915RNA PCR Primer, Index 15 (96% over 25bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1146110.20656545846543048Illumina Paired End PCR Primer 2 (100% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG1088190.19612643310633077Illumina Paired End PCR Primer 2 (100% over 24bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT1061020.1912295353334244TruSeq Adapter, Index 1 (96% over 29bp)
CCTGGAGGTACTGCAATACCAGGTCAATCTCGTATGCCGTCTTCTGCTTG999840.18020295433429254RNA PCR Primer, Index 15 (96% over 31bp)
TCAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTT992770.17892871556894663Illumina PCR Primer Index 12 (96% over 27bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG980070.17663977181790094RNA PCR Primer, Index 23 (96% over 29bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT977760.1762234363797186Illumina PCR Primer Index 2 (96% over 28bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTC975240.17576925226533788Illumina PCR Primer Index 12 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTG938930.16922503591884427RNA PCR Primer, Index 31 (95% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTT911110.1642109874815143No Hit
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC879100.15844176783813066RNA PCR Primer, Index 35 (95% over 24bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG873940.1575117717943987RNA PCR Primer, Index 15 (96% over 29bp)
GGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTC835370.15056023159929383No Hit
CGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGATCTCGTATGCCGTCTTC825060.14870204182974414RNA PCR Primer, Index 17 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCT769630.13871179363128255RNA PCR Primer, Index 15 (95% over 23bp)
CCTTCGAGCCGGAAACGAACCAGCGACCTATCTCGTATGCCGTCTTCTGC757430.13651296577594732Illumina Paired End PCR Primer 2 (96% over 26bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTATCTCGTATGCCGTCTT754940.13606418861530925Illumina PCR Primer Index 1 (95% over 21bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATTCCGTCTTCTGCTT753630.13582808496854784RNA PCR Primer, Index 46 (96% over 26bp)
CAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTTG705900.12722562156402734Illumina PCR Primer Index 12 (96% over 28bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCGCGTATGCCGTCTTCT677930.1221845383579842RNA PCR Primer, Index 23 (95% over 21bp)
ACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCT643060.11589985579113674Illumina PCR Primer Index 12 (95% over 23bp)
CCTGGAGGTACTGCAATACCAGGTCAAATCTCGTATGCCGTCTTCTGCTT631990.11390468986010716RNA PCR Primer, Index 13 (100% over 28bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT622970.11227899910149046RNA PCR Primer, Index 24 (96% over 28bp)
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG594420.10713338145642319RNA PCR Primer, Index 13 (96% over 31bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG588180.10600873507795666No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTA586330.10567530626382794RNA PCR Primer, Index 23 (95% over 21bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT572230.10313403800479297RNA PCR Primer, Index 13 (96% over 31bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTA555290.10008091145812259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)