FastQCFastQC Report
Thu 28 Sept 2023
SRR9651908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58021310
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT20471813.528326058132779RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC16993432.928825633202697RNA PCR Primer, Index 23 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT8696381.4988251730269448RNA PCR Primer, Index 8 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC8572691.4775071434960707No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT7789921.3425963667486998RNA PCR Primer, Index 3 (95% over 21bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4871370.8395829049706047TruSeq Adapter, Index 2 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA3584650.6178161092881219RNA PCR Primer, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2967530.5114551877577393Illumina PCR Primer Index 5 (95% over 21bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT2863500.4935255684506262TruSeq Adapter, Index 10 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTT2578260.4443643206263354No Hit
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG2561430.4414636622303081RNA PCR Primer, Index 35 (96% over 28bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT2491170.4293543182668575RNA PCR Primer, Index 1 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTC1998360.34441828355823056No Hit
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT1947850.33571286136076556RNA PCR Primer, Index 30 (95% over 23bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1930470.3327174102066982Illumina PCR Primer Index 12 (95% over 24bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT1766910.3045277674702622Illumina PCR Primer Index 9 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCTCGTATGCCGTCTTCT1714150.29543455671717855RNA PCR Primer, Index 17 (95% over 23bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT1619850.2791819074750294Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTT1608990.27731018137991026No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATTCCGTCTTC1583150.2728566452567169No Hit
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1475660.2543306933262969RNA PCR Primer, Index 35 (96% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1467860.2529863596668189Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTGCT1448080.2495772673867584RNA PCR Primer, Index 31 (96% over 26bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT1437570.2477658639558466Illumina PCR Primer Index 5 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG1419230.24460495635138194Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCTGC1346290.2320337131305722RNA PCR Primer, Index 15 (96% over 25bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG1270760.21901608219462815Illumina Paired End PCR Primer 2 (100% over 24bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT1265950.21818707643795013TruSeq Adapter, Index 1 (96% over 29bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG1150320.1982581916885365RNA PCR Primer, Index 23 (96% over 29bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATTCCGTCTTCTGCTT1065310.18360667830491936RNA PCR Primer, Index 46 (96% over 26bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT1049100.18081287719977368Illumina PCR Primer Index 2 (96% over 28bp)
CCTGGAGGTACTGCAATACCAGGTCAATCTCGTATGCCGTCTTCTGCTTG1036240.1785964501663268RNA PCR Primer, Index 15 (96% over 31bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTC1016610.17521321045664084Illumina PCR Primer Index 12 (95% over 22bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG1011320.17430147647476418RNA PCR Primer, Index 15 (96% over 29bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTG1009470.1739826281068111RNA PCR Primer, Index 31 (95% over 24bp)
TCAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTT996130.1716834728481656Illumina PCR Primer Index 12 (96% over 27bp)
GGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTC920170.15859173121048112No Hit
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC872210.15032580270938384RNA PCR Primer, Index 35 (95% over 24bp)
CGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGATCTCGTATGCCGTCTTC866990.14942613326034865RNA PCR Primer, Index 17 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCT820940.1414893941553543RNA PCR Primer, Index 15 (95% over 23bp)
CCTTCGAGCCGGAAACGAACCAGCGACCTATCTCGTATGCCGTCTTCTGC783440.1350262515617107Illumina Paired End PCR Primer 2 (96% over 26bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCGCGTATGCCGTCTTCT781910.13476255534389003RNA PCR Primer, Index 23 (95% over 21bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTATCTCGTATGCCGTCTT781650.13471774422190744Illumina PCR Primer Index 1 (95% over 21bp)
CAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTTG738800.12733252661823732Illumina PCR Primer Index 12 (96% over 28bp)
ACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCT704490.12141918202122634Illumina PCR Primer Index 12 (95% over 23bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT699650.12058500575047341RNA PCR Primer, Index 24 (96% over 28bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG673710.11611423458036366No Hit
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT665690.11473198381766975RNA PCR Primer, Index 13 (96% over 31bp)
CCTGGAGGTACTGCAATACCAGGTCAAATCTCGTATGCCGTCTTCTGCTT645090.11118156415289486RNA PCR Primer, Index 13 (100% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGAATCGCGTATTCCGTCTTC612560.10557500339099547No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGAATCGCGTATGCCGTCTTC588220.10137999297154787RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)