FastQCFastQC Report
Thu 28 Sept 2023
SRR9651909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9651909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56579456
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT18588643.285404511489117RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC16403602.8992148669651403RNA PCR Primer, Index 23 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT7097541.254437653129786RNA PCR Primer, Index 3 (95% over 21bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT6663841.1777843887364348RNA PCR Primer, Index 8 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC5978601.0566732914505221No Hit
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG4688090.8285852023745157TruSeq Adapter, Index 2 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA3234450.5716650934218951RNA PCR Primer, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT2703880.47789077364052424Illumina PCR Primer Index 5 (95% over 21bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT2648300.468067420089723TruSeq Adapter, Index 10 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG2412960.4264728172713432RNA PCR Primer, Index 35 (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTT2311330.4085104671207867No Hit
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT2168900.3833370190056264RNA PCR Primer, Index 30 (95% over 23bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT2069840.3658288973298011RNA PCR Primer, Index 1 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG1939410.34277636038070075Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG1741870.3078626277354098Illumina PCR Primer Index 12 (95% over 24bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT1726480.3051425591649379Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCTCGTATGCCGTCTTCT1546610.27335186821167035RNA PCR Primer, Index 17 (95% over 23bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT1408710.24897906406169756Illumina PCR Primer Index 9 (96% over 27bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT1341410.23708428727204447Illumina PCR Primer Index 5 (95% over 22bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG1307980.23117578224859567RNA PCR Primer, Index 35 (96% over 26bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTGCT1304450.2305518808805797RNA PCR Primer, Index 31 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATTCCGTCTTC1281770.22654335877672632No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTC1267490.2240194744891149No Hit
CGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCTGC1202020.21244813665228596RNA PCR Primer, Index 15 (96% over 25bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTT1144410.2022659956292263No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG1052140.18595795618819663Illumina Paired End PCR Primer 2 (100% over 24bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT1047210.18508661518414035TruSeq Adapter, Index 1 (96% over 29bp)
CCTGGAGGTACTGCAATACCAGGTCAATCTCGTATGCCGTCTTCTGCTTG973980.17214375479325925RNA PCR Primer, Index 15 (96% over 31bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG962730.170155400575078RNA PCR Primer, Index 23 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG955360.16885280763392282Illumina Paired End PCR Primer 2 (100% over 24bp)
TCAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTT940460.166219342936065Illumina PCR Primer Index 12 (96% over 27bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTC934310.16513237596345925Illumina PCR Primer Index 12 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTG914470.1616258028355734RNA PCR Primer, Index 31 (95% over 24bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT904840.15992377162481025Illumina PCR Primer Index 2 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG871910.15410363789994727RNA PCR Primer, Index 15 (96% over 29bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTATCTCGTATGCCGTCTT817160.14442698070479856Illumina PCR Primer Index 1 (95% over 21bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC809650.14309964380003937RNA PCR Primer, Index 35 (95% over 24bp)
GGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTC805390.14234672033608808No Hit
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATTCCGTCTTCTGCTT781880.13819150187658222RNA PCR Primer, Index 46 (96% over 26bp)
CGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGATCTCGTATGCCGTCTTC779770.13781857499654998RNA PCR Primer, Index 17 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCT752690.1330323854651413RNA PCR Primer, Index 15 (95% over 23bp)
CCTTCGAGCCGGAAACGAACCAGCGACCTATCTCGTATGCCGTCTTCTGC732340.12943567361269787Illumina Paired End PCR Primer 2 (96% over 26bp)
CAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTTG688890.12175620776558899Illumina PCR Primer Index 12 (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTA633400.11194876104853324RNA PCR Primer, Index 23 (95% over 21bp)
ACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCT630700.11147155603616975Illumina PCR Primer Index 12 (95% over 23bp)
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG628900.11115341936126075RNA PCR Primer, Index 13 (96% over 31bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGGGTATGCCGTCTTT623460.11019193963264687No Hit
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCGCGTATGCCGTCTTCT622930.1100982660561459RNA PCR Primer, Index 23 (95% over 21bp)
CCTGGAGGTACTGCAATACCAGGTCAAATCTCGTATGCCGTCTTCTGCTT613130.10836618860386356RNA PCR Primer, Index 13 (100% over 28bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT573200.10130885669879894RNA PCR Primer, Index 13 (96% over 31bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT568610.10049760817778099RNA PCR Primer, Index 24 (96% over 28bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG565840.10000803118361548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)