FastQCFastQC Report
Fri 14 Jul 2023
SRR9681161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6586696
Total Bases665.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1876802.8493800230039463No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1303621.9791713478199084No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA884281.3425243855189308Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG687851.0443020294241605No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG679631.0318223279167582No Hit
ACCGAACTGGGTGACGTTGCTGACCACTAGATCGGAAGAGCACACGTCTG533230.8095561112885732Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATG420850.6389394622129213TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTC412950.6269455885014277RNA PCR Primer, Index 38 (100% over 34bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATC382640.5809285869577099TruSeq Adapter, Index 3 (97% over 37bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC372030.5648203590996154No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA348740.5294612048286425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC323890.49173364005261516No Hit
ACCGAACTGGGTGACGTTGCTGACCACTACGAGATCGGAAGAGCACACGT319150.484537315825719No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG299610.45487145603805No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC295470.4485860589284825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA275210.4178270866000192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC238090.36147106227462145Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGGA236220.3586320060922805No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA233110.35391036720079383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA232030.35227069838960234No Hit
ACCGAACTGGGTGACGTTGCTGACCACAGATCGGAAGAGCACACGTCTGA230320.34967455610521575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA202360.3072253524377017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG199190.3024126208344821No Hit
ACCGAACTGGGTGACGTTGCTGACCAGATCGGAAGAGCACACGTCTGAAC192150.2917244093244929Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA190740.28958373059877063No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTAGATCGGAAGAGCA184580.28023154552753005No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC179670.27277712528405745No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC175730.26679537054693275Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT160110.24308090125914417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCACCAGCAGATGGGCGGCCTGCGCTCCTGTATGGGTCTGACCGGCTG149130.22641093501203033No Hit
TGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATCG144540.21944234256446632No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA143790.2183036836678055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA141590.21496361757093388No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT135430.20561143249969333No Hit
ACCGAACTGGGTGACGTTGCTGACCAAGATCGGAAGAGCACACGTCTGAA133930.20333411470637178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG130130.1975649096299571No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC120470.18289898304096622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC118830.18040911558693465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA115060.17468545686638642No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT112800.17125429805778192TruSeq Adapter, Index 12 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC108060.16405797383088577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC102470.1555711695211074No Hit
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA97810.1484963022431884No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG96440.14641635199195469Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA90570.1375044483607563No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA85280.12947310760964223No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC84920.12892655133924505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC82940.12592049185206058Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG81010.12299034295798683No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG80020.1214873132143946No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC71450.10847623755521738No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATC70120.10645701577847225TruSeq Adapter, Index 3 (97% over 37bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCT69910.10613819128740723TruSeq Adapter, Index 3 (97% over 37bp)
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAG69570.10562199925425433No Hit
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT69090.10489325756039145TruSeq Adapter, Index 12 (100% over 35bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA67920.10311694968160061No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA66500.10096108883725619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)