FastQCFastQC Report
Fri 14 Jul 2023
SRR9681162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7826377
Total Bases790.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA2718543.4735612659599706No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1871842.3917069162397877No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1060501.355033114300525No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1049221.3406203151215434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAACTGGGTGACGTTGCTGACCACTAGATCGGAAGAGCACACGTCTG703730.8991772310482871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG629200.8039479825722682No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATG607840.7766556607227073TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC481480.6152016443879461No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC416910.5326985909316662No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTC409250.5229111758863648RNA PCR Primer, Index 39 (100% over 34bp)
ACCGAACTGGGTGACGTTGCTGACCACTACGAGATCGGAAGAGCACACGT393410.5026719259754546No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA364310.46548997064669895No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA357200.45640530733441537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG332790.42521590769266543No Hit
ACCGAACTGGGTGACGTTGCTGACCACAGATCGGAAGAGCACACGTCTGA321280.41050923051624016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAACTGGGTGACGTTGCTGACCAGATCGGAAGAGCACACGTCTGAAC291670.3726756326714136Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA269610.34448889952528483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA255520.3264856778557946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG253110.32340634753475334No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC249470.31875540879259967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC239870.3064891967253814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTAGATCGGAAGAGCA232480.2970467688944706No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC219520.28048738260372585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC210350.268770594618685Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGGA202390.25859986044628314No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA197140.2518917757220231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCACCAGCAGATGGGCGGCCTGCGCTCCTGTATGGGTCTGACCGGCTG178590.22818987636297103No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT177520.22682270480964564No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATC175640.2244205716131487TruSeq Adapter, Index 2 (97% over 37bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA175200.22385837022673455No Hit
ACCGAACTGGGTGACGTTGCTGACCAAGATCGGAAGAGCACACGTCTGAA175180.22383281561826118Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG173620.22183955615733816No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA166090.2122182460671138No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA159040.20321024658025033No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT154880.19789488801778907Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT152730.19514776760690164No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC141220.18044109043047632No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA137460.1756368240374825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATCG137310.1754451644739322No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC136550.17447408935194408No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG127750.1632300616236606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC122460.15647086768245383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGAGATTCCGGACGAAGAAGCTGAGATCGGAAGAGCACACGTCTGAAC120730.1542603940495072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG108870.1391065112247979No Hit
AGTACCGAGGATGTAGGAATTAGATCGGAAGAGCACACGTCTGAACTCCA107960.1379437765392595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC98510.12586922403559142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT97730.12487259430512994TruSeq Adapter, Index 12 (100% over 35bp)
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA96460.12324987666707085No Hit
GGGGCTGATTCTGGATTCGACGGGATTAGATCGGAAGAGCACACGTCTGA94910.12126939451038456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG92930.11873948827152078No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA91800.11729565289277528No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA91450.11684844724449128No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC83400.10656271733395925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)