FastQCFastQC Report
Fri 14 Jul 2023
SRR9681163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10902902
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1600281.4677560157836878No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1476271.354015655648377Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1262751.1581778869515658No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1108821.0169952917122433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC834730.7656035062958467Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC794670.7288609949901411No Hit
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC742930.681405739499447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA708500.6498269910157864TruSeq Adapter, Index 7 (100% over 35bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC617980.5668032235821252Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA572120.524741027664011No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT525320.48181667596388555No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA516910.47410313327589293Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGG498290.4570251112960568No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG480100.44034147972714055No Hit
AGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGA386400.3544010576266759No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC343080.31466851669399576No Hit
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA342800.31441170433339677No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA268850.24658572552518587No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC264440.24254093084575098No Hit
TGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATCG263660.24182552498408222No Hit
ATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACGTC259980.2384502768162091No Hit
TCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACGTC253690.2326811705727521No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC251730.23088348404855882No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC248930.2283153604425684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC245470.225141893415166No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC243010.22288561338990298Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA240810.22086780198519623TruSeq Adapter, Index 7 (100% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA226620.20785291842483775No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA216730.19878193897367877No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA202820.18602386777391927No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG201280.18461139979062455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA196910.18060329259127525No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATC192280.1763567167713697TruSeq Adapter, Index 12 (97% over 37bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC186300.17087193849857588No Hit
TCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAGATCGGAAG186130.17071601670821218No Hit
TGATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACG182480.16736828415040325No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG181800.16674459698894845No Hit
ATCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAG179880.16498359794484074No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTAGATCGGAAGAGCA170150.1560593684140241No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT164840.15118910543266373No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG161690.14829996637592452No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG156140.14320957851405067No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA153420.14071482986823142No Hit
TGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGAGATCGGAAGAGCAC152050.13945828367530042No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG145830.13375338052199315No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG140500.12886477380058997No Hit
ATGCCGATCGGTTCGGGCTTAGGCTCCAGATCGGAAGAGCACACGTCTGA136910.1255720724629094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTAGATCGGAAGAGCA133570.12250866787576371No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTC133070.122050074374694RNA PCR Primer, Index 34 (100% over 34bp)
ACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGAAG130570.11975710686934543No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA128960.11828043579590095No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG124160.11387793818563169No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC122690.11252967329248671No Hit
TCATCGGTCACTCTGAACGTCGTACAGATCGGAAGAGCACACGTCTGAAC120360.11039262757750183Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACAC119890.10996154968649632No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAGATCGGAAGAGCAC115840.10624694232783162No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA115040.10551319272612007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC111740.10248647561905996No Hit
CTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGATCGGAA111490.10225717886852509No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG109350.10029439868394673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)