FastQCFastQC Report
Fri 14 Jul 2023
SRR9681164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19780663
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC5553882.8077319754145753TruSeq Adapter, Index 16 (100% over 35bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4109682.077625001750447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3047861.5408280298794839Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA2537721.282929697553616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC2121691.0726081324978844No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1955750.9887181233510727Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1535810.7764198803649808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA1497420.7570120374630517No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATC1426710.7212650051214158TruSeq Adapter, Index 2 (97% over 36bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT1272660.64338591684212No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC1062080.5369284133701686TruSeq Adapter, Index 16 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC940220.47532279378097686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC897060.4535035049128535No Hit
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG890640.4502579109709316No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG871310.4404857410492257No Hit
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA762190.38532075492110657No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC707960.35790509145219246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAGATCGGAAGAGCACACGTCTGAA645900.3265310166802801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA627970.3174666086773735No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG618530.3126942711677561No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA591810.299186129403246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG540910.27345392821261855No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA532340.26912141418111213No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTC525720.26577471139364744RNA PCR Primer, Index 36 (100% over 34bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCT503090.25433424552048634TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC435040.21993196082456895No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA429490.2171261903607579Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA355980.17996363418152364No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATC355520.17973108383677533TruSeq Adapter, Index 2 (97% over 36bp)
AGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGAGATCGGA343140.17347244629767972No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT337070.17040379283545753No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG335570.16964547649388698Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAAC332170.16792662611966042Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGGA331770.16772440842857492No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA318370.1609501157772113No Hit
TTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC315270.15938292867129883Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCA310750.15709786876203288TruSeq Adapter, Index 23 (100% over 36bp)
GCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAT304260.153816886724171TruSeq Adapter, Index 2 (97% over 36bp)
AAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGG303900.15363489080219406No Hit
AGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGA299660.15149138327668793No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC296420.14985341997889556No Hit
ACTGAGATTCCGGACGAAGAAGCTGAGATCGGAAGAGCACACGTCTGAAC288230.14571301275392032Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA284090.1436200596511856No Hit
ATCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAG281960.14254325044615543No Hit
CGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAACT280770.14194165281517612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA251450.12711909605861035No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC249810.1262900035251599No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC246370.12455093138182476No Hit
TGTGGGCACTCGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA241470.12207376466602762Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA240830.12175021636029086Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATG235400.11900511120380546TruSeq Adapter, Index 2 (97% over 36bp)
TCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACGTC235100.11885344793549134No Hit
CGTGGACTGTCTCTGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT232910.11774630607679834Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGAGATCGGAAGAGCACAC230740.1166492751026596No Hit
AGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCA229020.11577973903099203Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA217070.1097384855098133No Hit
ACTGCTGCCGTGGATCGACGGTCTGTTGGACGCGGGAGATCGGAAGAGCA215560.1089751137259656No Hit
CGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA211150.10674566368174818TruSeq Adapter, Index 2 (97% over 36bp)
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC210050.1061895650312631No Hit
ACCGAACTGGGTGACGTTGCTGACCACTAGATCGGAAGAGCACACGTCTG208690.10550202488157248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCCGATCGGTTCGGGCTTAGGCTCCAGATCGGAAGAGCACACGTCTGA206510.1043999384651566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC201350.10179133025015391Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)