FastQCFastQC Report
Fri 14 Jul 2023
SRR9681165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10158357
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1351041.329978853863868No Hit
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1227251.2081185963438772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA1201261.1825337502905244No Hit
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1018141.0022683786364273TruSeq Adapter, Index 7 (100% over 35bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC974190.9590035081460515Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC855980.8426362648999243No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA749340.7376586587772018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC675320.6647925446998959Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT611190.6016622569968746No Hit
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA584280.5751717526761463No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC441870.4349817593534072No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA412810.4063747710382693No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG405100.3987849609932No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA389820.38374315846548807No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC375700.3698432728835972No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA365740.36003853772809913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA308690.30387788103922714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTC298330.2936793814196528RNA PCR Primer, Index 35 (100% over 34bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC274280.2700042930170696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG272080.2678385884646503No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC269760.2655547545730082No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATC265310.2611741249101602TruSeq Adapter, Index 9 (97% over 36bp)
TGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGAGATCGGAAGAGCAC259580.2555334489622682No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAGATCGGAAGAGCACACGTCTGAA255810.25182221888834977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA248810.24493134076701578TruSeq Adapter, Index 7 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC232300.22867871251226946Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG202000.1988510543584952TruSeq Adapter, Index 9 (97% over 36bp)
ATCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAG196340.19327928719181656No Hit
AAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGG188850.18590604760198917No Hit
TCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACGTC187650.18472475420976048No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA171620.16894464331190565No Hit
AGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGA168710.16608000683575108No Hit
TCATCGGTCACTCTGAACGTCGTACAGATCGGAAGAGCACACGTCTGAAC167250.16464276654187285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACGTCTGAAC152190.14981753446940288Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC149660.14732697423412072No Hit
ATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACGTC147800.14549596947616628No Hit
ATGCCGATCGGTTCGGGCTTAGGCTCCAGATCGGAAGAGCACACGTCTGA146580.14429498786073378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA145540.1432712002541356No Hit
ACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGAAG144780.14252304777239075No Hit
TGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATCG141810.13959934662662474No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT138190.1360357782267349No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC129560.12754030991429027No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA129300.12728436301264073Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCTGCCGTGGATCGACGGTCTGTTGGACGCGGGAGATCGGAAGAGCA128150.12615229017842156No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTAGATCGGAAGAGCA127760.12576836982594725No Hit
ACGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAAC125700.12374048283595467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC123750.12182088107358306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA123670.12174212818076781No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA121610.11971424119077524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA119280.1174205631875312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGACGTTGACTCCTCTGAACTGGCGAGATCGGAAGAGCACACGTCTGAA115360.11356167143958418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACGTCTGA111630.10988981781207335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGAGATCGGAAG109420.10771426914805218No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC106990.1053221500287891No Hit
TCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAGATCGGAAG105440.10379631273049372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)