FastQCFastQC Report
Fri 14 Jul 2023
SRR9681166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622185
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA3365642.154397736296171Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2785251.7828812038776904No Hit
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG2063111.3206283243989239No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC1809201.1580966426911472No Hit
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA1564211.0012747896661063No Hit
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1077350.6896282434243353Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1005190.6434375217039102TruSeq Adapter, Index 2 (100% over 35bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC892760.5714693559191625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC783970.5018312099107776No Hit
TCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGGA708870.4537585491402131No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC578730.3704539409820073Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT538740.34485572920817414No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG536140.3431914293679149No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA480880.3078186566091747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA479800.307127332060144No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG476580.30506616071951526TruSeq Adapter, Index 2 (100% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC471520.3018271771842415No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG447970.286752461323432Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC433060.27720834185486853No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC423630.271172054357313Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA397830.25465707901935614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG375020.24005604849769735No Hit
TCGAGCAGGCGAACAAACGCCGCTCGCAGATTCTGGACGAGATCGGAAGA354130.2266840393965377No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT351910.22526298337908557No Hit
AGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGAGATCGGA349700.22384832851486525No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAGATCGGAAGAGCACACGTCTGAA334070.2138433260136146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC329400.21085398745437975Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGAGATTCCGGACGAAGAAGCTGAGATCGGAAGAGCACACGTCTGAAC326470.20897844955747227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGACGTTGACTCCTCTGAACTGGCGAGATCGGAAGAGCACACGTCTGAA303040.19398054753544397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA298920.1913432724039563No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA298850.19129846433133393No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG264620.1693873168189981No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATC260610.16682045437305984TruSeq Adapter, Index 7 (97% over 37bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA226390.14491570801395579Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAGATCGGAAGAGCA219310.1403836915258653No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTAGATCGGAAGAGCA205640.13163331505804088No Hit
ATGACGTTGACTCCTCTGAACTGGCGTAGATCGGAAGAGCACACGTCTGA203750.13042349709723705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA203270.13011624174211225No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA202050.12933530104783678Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGATCGGAAGAGCACAC196200.12559062640725352No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA185700.11886941551389898No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA180210.11535518238965932No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA174980.11200737924944558No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA174270.11155289736999018No Hit
TGTGGGCACTCGAAGATACGGAAGATCGGAAGAGCACACGTCTGAACTCC167120.10697607280927733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC165390.10586867330018175Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG164980.10560622601767936No Hit
ACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGAGATCGGAAGAGCACAC159510.1021047951999032No Hit
AGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCA159200.10190635944971844Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATGTGAACGCTTCCGACGAAGATCGTTGGAGATCGGAAGAGCACACGT157580.10086937262617232No Hit
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGAGATCGGAAG157180.10061332649690168No Hit
ACTGCTGCCGTGGATCGACGGTCTGTTGGACGCGGGAGATCGGAAGAGCA156310.10005642616573802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)