FastQCFastQC Report
Fri 14 Jul 2023
SRR9681167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10327217
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2187762.1184410088410073No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1813551.756087821142908Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1757421.7017362954608195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1331751.2895536135243406No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1205841.1676330612593888No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA997130.9655360200139108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC718470.6957053386212375Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTAGATCGGAAGAGCA663180.642167197609966No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC635900.6157515621101018Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCAGATCGGAAGAGCACACGTCTGA616890.5973438923574473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA568070.5500707499416349No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC542520.5253302995376199Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG531690.5148434471745873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA518720.5022844005311402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA487810.4723537812752458No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT440530.42657184408926435TruSeq Adapter, Index 12 (100% over 35bp)
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC379100.3670882484603548No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA377610.36564545898473905No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCAGATCGGAAGAGCACACGTCTGAA371780.36000018204323586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC363070.35156615765893173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA344130.3332262699621786No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC309770.29995496366542895No Hit
CGGACGTGGGGGTTCAAGTCCCCCCAGATCGGAAGAGCACACGTCTGAAC304520.29487130947282314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG287200.2781000922126455No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC258740.25054184491330045No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG256860.24872141255480543No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC256200.24808232459916355No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCAGATCGGAAGAGCACACGTCTGAA242500.23481640794417316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT239860.23226005612160563No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC232690.22531723696713254No Hit
TGTGGGCACTCGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA232590.225220405458702Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT219550.21259357675935345TruSeq Adapter, Index 12 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG218960.21202227085961298No Hit
ACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGAAG216630.20976609671318033No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG211030.20434353224106747No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC206130.1995987883279687No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATG196040.1898284891273225TruSeq Adapter, Index 3 (97% over 37bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA193990.18784344320449545No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA192610.18650716838815337No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG189670.18366032204029412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAACT176410.17082046402239828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG173480.1679833008253821Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT169860.16447800022019485No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA167950.16262851840917064No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA167720.1624058059397803No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC166280.16101143221837985No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG162610.1574577158589773No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA161630.15650876707635755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG159680.15462055266196112No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA155190.15027281793342775TruSeq Adapter, Index 2 (97% over 36bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT154740.14983707614549013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGAGATCGGAAGAGCAC144310.13973754981617992No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC138080.13370494684095435Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTC126420.12241439295794791RNA PCR Primer, Index 38 (100% over 34bp)
TGTGGGCACTCGAAGATACGGAAGATCGGAAGAGCACACGTCTGAACTCC122820.11892845865444678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAAC121170.1173307387653421Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG119300.11551998955769012No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTAGATCGGAAGAGCACACG118070.11432896200399391No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG112970.10939055507403399No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA110280.10678578749725119No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTAGATCGGAAGAGCACAC109810.10633067940762743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)