FastQCFastQC Report
Fri 14 Jul 2023
SRR9681168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14703557
Total Bases588.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACT3866292.6294929859489105Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAAC3281962.232085746326552Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGA1342950.9133504226222268No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAA1324660.9009112556913949Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGA1127440.7667804463913052TruSeq Adapter, Index 1 (97% over 37bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACT822650.5594904688708997Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610470.41518525075259Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAA592850.40320175587444596No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACAC570330.38788573404380994No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAA547320.3722364595179248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGC536260.3647144701108718No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCC492750.335122990987827No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAAC410050.2788780973202607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGG405140.2755387692923556No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGT371430.25261234407429445No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAGAT360920.24546441381497006No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAA335920.22846172528184847No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC324120.22043645629421507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCG313310.21308449377249328No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGA310620.2112550044863294No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGC309350.2103912679088468No Hit
AGGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC300900.20464435918465174Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC284080.1932049503395675No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGA238110.16194040666486348No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGC228160.15517333662868107No Hit
TGGCTCCGGATCGTCGCGTAGAGATCGAGATCGGAAGAGC227050.15441841725781047No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCA219770.14946723435696546No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAAC212560.1445636589840132Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGA210650.1432646535800827No Hit
ACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGA206510.14044900835899776No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGAC204610.13915680403048053No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAG204550.13911599757800103No Hit
TGCCTGGCTCCGGATCGTCGCGTAGAGATCGAGATCGGAA202330.13760615883625982No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAGATC199870.13593309428460065No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATC175850.1195969111419774No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA174570.11872637348908158Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAG171390.11656363150766853No Hit
GCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC161150.1095993302845019Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGTTCTTACCATGACGTTGACTCCTCTGAACTGGCGTT157040.10680408828965672No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTA155320.10563430331857794No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAG150040.10204333550038265No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTT147320.10019344298797903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)