FastQCFastQC Report
Fri 14 Jul 2023
SRR9681169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9745331
Total Bases984.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2670442.740225036994639Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1854021.9024700135890713TruSeq Adapter, Index 12 (100% over 35bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1644531.6875055347016947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1386261.4224863167808255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1241301.2737381624082342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC944900.9695925156364622Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC694100.7122385068295781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATC611130.6271003006465352TruSeq Adapter, Index 2 (97% over 37bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA556290.5708271991992884Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT447290.45897876634462187TruSeq Adapter, Index 12 (100% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTC425880.43700927141417767RNA PCR Primer, Index 39 (100% over 34bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCT352530.36174245902986774TruSeq Adapter, Index 2 (97% over 37bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA344200.35319477604198357TruSeq Adapter, Index 2 (97% over 36bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT338700.34755104777867474No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC324890.33338015917571195Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG306020.314017040570505No Hit
TGTGGGCACTCGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA297930.3057156293613834Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACGTC291540.29915864325183006No Hit
CCCGTGGCGTAACTGTAGATAGATCGGAAGAGCACACGTCTGAACTCCAG258740.265501500154279Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG258680.2654399322095884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT255790.2624744095403224No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC233730.23983792854239636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGAAGACGGTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT213040.21860724894823994Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGGCACTCGAAGATACGGAAGATCGGAAGAGCACACGTCTGAACTCC211410.21693465311747748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGATCGGAA170090.17453486187385528No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG159670.16384256214591375Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGACTGTCTCTGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT152470.1564544087830367Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACGT149440.1533452275761593No Hit
AGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGA130940.1343617779632113No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC130390.13379740513688043No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC129860.13325355495877975No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC128330.1316835723691684No Hit
AAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGG125900.12919007060919738No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAGATCGGAAGAGCA117340.12040637716666577No Hit
TGCTAACCAGCGTCTGGACAACATGGCTAGATCGGAAGAGCACACGTCTG113690.11666099386465169Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGAAG113230.11618897295535677No Hit
TCATCGGTCACTCTGAACGTCGTACTAGATCGGAAGAGCACACGTCTGAA112680.11562460012902588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATCCCGAAAGATTCGGTGATCGGTCTGTCAAAGATCGGAAGAGCACACG112650.11559381615668057No Hit
ATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACGTC111460.11437271858698284No Hit
TGATGTTATCGCCTGTATGAACCTGAACGGTGAGATCGGAAGAGCACACG110600.11349024471308364No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA108210.11103778824957304No Hit
GGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGAGATCGGAAGAGCAC106650.10943702168761636No Hit
CGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAACT104250.10697430389999067Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTACTGTAGAGTCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG103580.1062867951842785Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG99750.10235670804819251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)