FastQCFastQC Report
Fri 14 Jul 2023
SRR9681170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236245
Total Bases609.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAAC2078891.3644372350273968Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACT1979401.299138993892524Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAA1918151.2589388002096318No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGA1455950.9555832162058303No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAA960020.6300896316644947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAA931190.6111676466215922No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCG807110.529730258341212No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCC648610.4257020020352784No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAA519290.34082544616472105No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACT518870.34054978769375266Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCG480950.3156617657434624No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGC475380.31200600935466716No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGG456860.299850783444346No Hit
ACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGA429820.28210362855152304No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAGAT429090.28162450787579224No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCG396660.2603397359388747No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGA381640.2504816639532903No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTAGAT374730.2459464257761673No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGG367160.24097801000180818No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGC320860.2105899452260055No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAC311180.20423667379987653TruSeq Adapter, Index 6 (97% over 36bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGA310330.20367879356101193No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAG308310.20235300758159247No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA303240.19902541603918816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAAC298230.19573720427835073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGC286780.1882222292959978No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAA283370.18598414504361147No Hit
CTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC280570.1841464219038221No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACAC270630.17762250475756985No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGT254020.16672086856046225No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAA249040.1634523466904083No Hit
TGGCTCCGGATCGTCGCGTAGAGATCGAGATCGGAAGAGC242270.15900899467027474No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAG231980.15225536213154883No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAA228070.14968911303277152Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGAGATC211800.13901062893120977No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGA209240.13733042491768807No Hit
TGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGA201200.13205353418772145No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCG199630.13102309657005384No Hit
TGCCTGGCTCCGGATCGTCGCGTAGAGATCGAGATCGGAA197830.1298417031230464No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAG195210.12812211932795778No Hit
CAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTAG191030.12537866121212937No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAGATC190560.12507018625652186No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCA189960.12467638844085273No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAG186400.12233985473454909No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGAT185330.12163758196327246No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGAGATCGGAAGAGC185320.12163101866634464No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTAGATCGG178690.11727955280320052No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTAGAT173600.11393883466694057No Hit
ACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGA172920.11349253047584888No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGAC171170.11234395351348052No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTAGATCGGA169510.11125444622346253No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAA162920.1069292335480297No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTA159230.10450737698166446No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAG157090.10310283143911114No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTT155840.10228241932313376No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAG153260.10058908871575642No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)