FastQCFastQC Report
Fri 29 Sept 2023
SRR9681747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9681747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367853611
Total Bases55.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5243162214.253393315201137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG68976611.8751103139232197No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT36793941.0002332150546702Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC26669980.7250161260480327No Hit
TTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC26330920.7157988725031165No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA24200580.6578861611338104No Hit
TCTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAG22406030.6091018092520505No Hit
TCGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA20675090.5620466778563171No Hit
TTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCA14840560.4034365724902453No Hit
AAATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAGC13773310.3744236725733813No Hit
ACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA13611560.3700265429771736No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA12957720.3522520810594952No Hit
AAAATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAG10457600.2842870013310811No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG10318300.2805001688565727No Hit
CTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC10261150.27894656170712434No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9540020.2593428395079694Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA9318290.253315169984834No Hit
AATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAGCA8804730.23935418157414798No Hit
AACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAG8730740.23734278362160754No Hit
AATCCTCTATCACCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCA8588260.2334695037151613No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8034680.21842058252895605Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCAGTGGGTCGGAGGTTCGACTGTAGGCACCATCAATAGATCGGAAGAG8023030.21810388045912102No Hit
AGGAGCGGTAGTTCAGTCGGTTAGACTGTAGGCACCATCAATAGATCGGA7895010.21462369170544854No Hit
CTGTAGGCACCATCAATCCTGTAGGCACCATCAATAGATCGGAAGAGCAC7861370.21370919748834546No Hit
AGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGA7678920.20874934404273116No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCC7434940.20211681434330137Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAGTCCACTCAGGCCTACCACTGTAGGCACCATCAATAGATCGGAAGAGC7273310.19772294691433653No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCCAGTC7141950.19415196117240235Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAG6982940.18982931772824163No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC6730860.18297659173991362No Hit
AGTCCCGTCCGTTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA6575630.1787567065639054No Hit
AGGGGCTATAGCTCAGCTGGGAGACTGTAGGCACCATCAATAGATCGGAA6358890.1728646888286221No Hit
CTGTAGGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6293120.1710767493322228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAGGGGTCGGAGGTTCGACTGTAGGCACCATCAATAGATCGGAAGAG6172430.1677958246276397No Hit
AGACCGCCAGCTAAGGTCCCAACTGTAGGCACCATCAATAGATCGGAAGA6160600.16747422930694025No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6008040.16332692735208734No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA5954390.1618684667472246No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT5826100.15838093811725557No Hit
TCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC5656570.15377231134479744No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC5437870.1478270115445462No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG5170230.14055129120371745No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5043590.13710861737333876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA5041490.13705152944658738No Hit
CGGTGGTTCAAGTCCACTCAGGCCTACCACTGTAGGCACCATCAATAGAT4932000.13407507368467833No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT4886440.13283653752144353No Hit
AATCCTCTCTCGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCA4695240.12763881771436517No Hit
CCTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAG4695020.1276328370744198No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG4559830.1239577338279819No Hit
TAGGCCGGGTGTGTAAGCGCAGCGACTGTAGGCACCATCAATAGATCGGA4473680.12161576959482397No Hit
CGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGAG4431380.12046585564168895No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACATCAATAGATCGGA4346260.11815189167736619No Hit
CTGTAGGCACCATCAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC4239290.11524394142755881No Hit
GGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGAG4208730.11441317616969104No Hit
ACCGGAGAATGTTATCACGGGAGACACCTGTAGGCACCATCAATAGATCG4126150.11216826141201046No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA4027730.10949274057826226No Hit
TGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGA3937130.10702980430984542No Hit
AAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3698430.1005408099691048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)