FastQCFastQC Report
Fri 14 Jul 2023
SRR9691451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937063
Total Bases299.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC406521.0325463422861152No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA358870.9115170369384487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC343200.8717157942354491Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG325130.8258186368874463No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA279850.7108090472517203No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG279260.7093104682348238No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC263620.6695854244648867Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA220110.5590715718798506No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT212760.5404028332795284Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC211550.5373294763126727No Hit
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC198030.5029891571458217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG192700.4894511467050438No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA179090.4548822307390052No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC164790.41856073931252813No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC164730.41840834144640304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC155710.3954978622389329No Hit
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC154560.39257690313820226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG148140.37627033146281885No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA141770.3600907580092063No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG139890.35531562487062057No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA139700.35483303162789115No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA137950.3503880938659097No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA128670.3268172239052309No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA118530.3010619845300926No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA116580.29610905388102754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT112720.28630479116031415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA107300.27253818392034873Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC106350.27012521770670167No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG101320.25734919659654926No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA98920.2512532819515461Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA92670.23537850423018375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA92060.23382912592457883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG91750.23304173694959923No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT88070.22369466782726108No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA81770.20769289188412782Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC79820.20273996123506277Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT69770.17721331865911213No Hit
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC69110.17553694213173626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC65890.1673582566496904No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA55200.14020603683507224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC53610.13616749338275766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA53360.13553250227390318No Hit
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC52980.13456731578844433Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC51830.13164635668771366No Hit
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA50940.12938578834019165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC49640.1260838345741483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT47850.12153729823475012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA47260.1200387192178535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT46970.11930212953158231Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT46450.11798134802516495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA45340.11516198750185098No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC45230.11488259141395503No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT44750.11366340848495439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC44170.11219022911241197No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA44050.11188543338016181No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA43660.11089484725034879Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC43490.11046305329632773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA41190.1046211350948664No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC40780.10357974967634502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)