FastQCFastQC Report
Fri 14 Jul 2023
SRR9691452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841586
Total Bases291.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC396741.0327505358463926No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA350660.9128000778844988Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC334450.8706039640919141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG317880.8274707373465022No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA271650.7071298156542637No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG266210.692968997700429No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC261020.679458952630502Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA214410.5581288561547236No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT210280.547378088112566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC209040.5441502546083831No Hit
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC193250.5030474392607637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG189080.49219254755718084No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA173760.4523131852313081No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC160720.41836887160667496Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC160440.41764000597669815No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC152180.39613846989238294No Hit
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC149940.3903075448525687Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG145070.3776304890740439No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA138300.3600075593778195No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA134890.35113101724131646No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG134860.35105292449524755No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA132130.34394648460297383No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA123480.32142974281976244No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA117750.30651402832059466No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA115280.30008439222758515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT110520.28769367651797983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC102810.2676238407782619No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA101540.26431791452801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG96070.25007900382810644No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA94240.245315346317901Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA90120.2345906091910997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA89410.23274241420080144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG89010.2317011775865489No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT82400.21449474253602546No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA80420.20934062129547537Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC79740.20757051905124602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT68140.17737465723792206No Hit
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC66690.17360017451125653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC61530.16016822218739865No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA53750.13991617004018653Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC53000.137963851388463Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA51240.1333824102857518No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC50810.1322630809254303Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC49220.12812416538377638No Hit
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA49190.12804607263770745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC48010.12497442462566241Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT45750.11909143775513552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT45630.11877906677085974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT45530.1185187576172966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC44650.116228037065941No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA44380.11552520235132054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC44370.11549917143596422No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT44050.11466618214456216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC43670.11367700736102224Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA42670.11107391582539086No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA42460.11052726660290828No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA42030.1094079372425868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40130.10446206332488718No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC39180.10198912636603737Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)