FastQCFastQC Report
Fri 14 Jul 2023
SRR9691453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3962611
Total Bases301.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC401261.0126151671208705No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA353680.8925428208824939Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC339590.8569854573158959Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG326550.8240778617936506No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG279460.7052420739759719No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA275470.6951729554074322No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC259840.6557292653757838Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA218390.5511265173391988No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT213720.539341358513364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC213300.538281451295623No Hit
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC196530.4959608702443919Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG192930.48687595123518307No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA177820.4487445272826427No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC164920.41619023416631107No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC162180.40927560136485763Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC155330.3919890193612242No Hit
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC152830.3856800478270514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG147410.3720021975409648No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA144080.3635986474574466No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG139980.35325193414140327No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA137520.34704390615177716No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA137290.3464634807706333No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA130510.3293535499699567No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA119610.3018464340809633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA119380.30126600869981934No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT112370.2835756525179989Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC104150.26283175411363874No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA102080.2576079256833436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG101690.2566237261240127No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA96980.24473762375363112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA91190.23012604568048695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG91160.23005033802207686No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT90400.22813241067568832No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA89890.22684538048271707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA79770.2013066637123856Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC77660.19598189173754377Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT68530.1729415276947447No Hit
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC67910.17137690275426987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC66130.1668849150219388No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA55250.1394282709052188No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA54680.13798982539542742Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC52660.1328921763958158No Hit
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC51440.12981339828713948Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC51160.12910679347531212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA49410.12469051340139115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC48670.12282305782727601Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT48090.12135937643134792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT46170.1165140862931032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT45940.11593366091195931Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT45780.11552988673377226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA45600.11507564078331181No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA44940.1134100722982902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC44250.1116687961548585No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA44230.11161832438258512No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA44010.11106313488757791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC43260.10917044342732608No Hit
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC43100.10876666924913901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC40560.10235675417041945Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40420.10200345176450579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)