FastQCFastQC Report
Fri 14 Jul 2023
SRR9691459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450103
Total Bases338.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC694411.5604357921603162No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA453981.020156162677583No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA357040.8023185081334072Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA281650.6329066990134835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC255670.5745260278245244No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG249620.560930836881753No Hit
AAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAACT233210.5240552859113597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA222700.5004378550339172No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA216780.4871347921609904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT209710.4712475194394377No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA209000.469652050750286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA208170.46778692538127764No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG184010.4134960471701442No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG162000.3640365178064418No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG155320.34902562929442305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAA142170.31947575146013474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCTCGTATG136810.30743108642653894TruSeq Adapter, Index 4 (97% over 37bp)
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAAAGATCGGAAGAGCA125550.2821283012999924No Hit
TGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTC120060.26979150819655184No Hit
ATCTGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACAC113610.2552974616542583No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA95000.21347820488649363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG87440.19648983405552636TruSeq Adapter, Index 4 (100% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA86090.19345619640713935No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC80720.18138905998355542No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA79540.1787374359649653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGTCTGGATACCATTATGAAAGATCGGAAGAGCACACGTCTGAACTCC76910.17282746039810765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC76780.17253533232826296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC76380.17163647672874088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA75030.16860283908035387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA74410.16720961290109465No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62370.1401540593554801No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA61270.13768220645679435No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC60830.1366934652973201No Hit
ACTCGGCTGCGAACCGAGCGGTCGGAGGTTCGAGATCGGAAGAGCACACG60490.1359294380377263No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC58370.1311655033602593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG56750.12752513818219488No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC56420.12678358231258915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54530.12253648960484735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA54210.12181740512522969No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT53260.11968262307636476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA52390.11772761214740424No Hit
ATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAGATCGGAAGAGCACA51830.11646921430807332No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG49270.11071653847113201Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA48860.1097952114816219No Hit
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAATGAGATCGGAAGAG48860.1097952114816219No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA48570.10914354117196837No Hit
ATGCGCGCGGCGATACCGAAGAACTTACCGAGATCGGAAGAGCACACGTC48390.10873905615218343No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC47670.10712111607304371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA46730.10500880541416682No Hit
AGGACGGGGATCAAGAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG46240.10390770730475227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCCGTCGGAAGCACTGACAGCGGTTTCTGAGATCGGAAGAGCACACGT45870.10307626587519436No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA45700.10269425224539747No Hit
TGCCTGAAAGTCTGGATACCATTATGAAAGATCGGAAGAGCACACGTCTG45620.10251448112549306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA45460.10215493888568422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG45220.10161562552597096No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC44740.10053699880654449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)