FastQCFastQC Report
Fri 14 Jul 2023
SRR9691463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4503609
Total Bases342.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1831404.066516431599635No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG768701.7068533258548866No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC706661.5690971396495568No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC625651.3892191795513331No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA534851.187603097871063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA386920.859133197397909Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA374380.8312888618883211Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA213760.47464155969134975No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA194390.4316316092271776No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA187160.4155778177013147No Hit
AAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAACT185280.41140338781630464Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC172480.3829817375353855No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT165580.36766069168082754No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA160580.3565584845398435No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG142050.31541370487535664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC140430.3118165897616778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA139310.30932969536209737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA130700.2902116946653229No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG129920.28847975035132933No Hit
TGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAA128670.2857041985660833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG125580.2788430345529552No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA114070.25328575371441Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA109180.24242779513052753No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG105730.23476727220324853No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT100430.22299893263380546No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA98730.2192241822058709No Hit
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAAAGATCGGAAGAGCA93600.20783331767922128No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA93400.20738922939358193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG93240.2070339587650704No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA79460.1764362758845184No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT74190.16473454955792122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACAAATAGCTGGTGGAATATATGACTATCAGATCGGAAGAGCACACGTC72750.1615371139013178No Hit
TGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTC72000.1598717828301702No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC71880.1596053298587866No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG70250.1559860103308258No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA68230.15150071864586823No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA65450.1453278914754811No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC64740.14375137806146138Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA64200.1425523396902351No Hit
ATCTGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACAC63570.1411534615904711No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA63560.14113125717618913No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA60210.13369277839172983No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG58750.13045093390656248TruSeq Adapter, Index 4 (97% over 37bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA58110.12902985139251652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAATGAGATCGGAAGAG57010.12658736582150004No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA56090.12454455970755898Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT54000.11990383712262764No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG53280.11830511929432595No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA51220.11373100995224052No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC51060.11337573932372903Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG50920.11306487752378148Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA50440.11199906563824702No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC50230.11153277293832568No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG49450.10980082862433217No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA49240.10933453592441085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT47780.1060926914392435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA47080.10453838243950574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA46930.10420531622527622No Hit
TTTGCAGTTGGTGCAACCTTTACCGAAGATCGTCATCAGATCGGAAGAGC46850.10402768091102048No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA46720.10373902352535488No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA46560.1033837528968434No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAAGATCGGAA45210.1003861569687777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)