FastQCFastQC Report
Fri 14 Jul 2023
SRR9691464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205751
Total Bases319.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1692664.024631986059089No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG717681.706425320947436No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC665861.5832130813260223No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC561041.3339829200539928No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA496911.1815012348567473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA360930.8581820464406951Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA340790.810295236213461Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA199630.4746595792285373No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA182310.4334778735117699No Hit
AAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAACT169130.4021398318635601Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA167790.398953718372771No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC158350.37650826213915184No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT153280.3644533402001212No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA142040.337728030023651No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG131510.31269088445797194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC130880.31119293557797406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA126450.3006597394852905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG122340.2908874063157805No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA120310.2860606821468984No Hit
TGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAA119450.2840158630408695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG118910.2827319068580142No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA109070.2593353719704281No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA101800.24204951743457948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG99190.23584372921744537No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT94330.22428812357174738No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA89650.21316050332033445No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG87560.20819111735335735No Hit
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAAAGATCGGAAGAGCA85640.20362593981431615No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA85610.20355460891526866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA73210.17407117064229433No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG67400.16025675319342492No Hit
AACAAATAGCTGGTGGAATATATGACTATCAGATCGGAAGAGCACACGTC67160.15968610600104474No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT65840.15654754644295396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA65530.1558104604861296No Hit
TGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTC65250.15514470542835274No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC64550.15348031778391064No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA61950.1472983065331257No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC60590.1440646391096382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA60220.14318489135471882No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA58070.13807284358964667No Hit
ATCTGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACAC57500.1367175565077438No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG55300.13148662391092578TruSeq Adapter, Index 4 (97% over 37bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA53910.12818162558839075No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA53750.127801194126804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAATGAGATCGGAAGAG51450.12233249186649423No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG50630.12038278062586207No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA50440.11993101826522777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT49330.1172917750004696No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG48990.11648335814459772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC48940.11636447331285185No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA48050.11424832330777547No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA46300.11008735419667022No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG45570.10835163565318061No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC45270.10763832666270542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT44540.1059026081192158Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA44470.10573616935477159Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA44290.10530818396048648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGCAGTTGGTGCAACCTTTACCGAAGATCGTCATCAGATCGGAAGAGC43980.10457109800366213No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA43230.10278782552747417No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA43140.10257383283033161No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG43120.10252627889763327Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA42590.1012660996811271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)