FastQCFastQC Report
Fri 14 Jul 2023
SRR9691465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089692
Total Bases310.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1654494.045512473799983No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG699791.7111068510782719No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC645941.579434343710969No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC546801.3370200005281572No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA490471.1992834668234185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA342300.8369823448807392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA341520.8350751107907393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA192020.46952191020741907No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA174980.42785618085665134No Hit
AAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAACT167220.4088816468330623Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA158400.3873152305846015No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC156220.3819847558202427No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT149040.3644284215046023No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA138480.3386074061322955No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG128450.3140823318724246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC124330.30400822360216856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA122120.2986043936805021Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTCTGAA118930.2908042952867845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA116180.28408007253357953No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG114830.28077909045473354No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG114130.27906747011755406No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA102820.2514125758125551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA97450.23828200265447863No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG97400.2381597440589658No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT94360.2307264214517866No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA87300.21346350776537695No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA85800.20979574989999247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG84370.2062991540683259No Hit
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAAAGATCGGAAGAGCA83690.20463643716935165No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA71930.1758812155047373No Hit
AACAAATAGCTGGTGGAATATATGACTATCAGATCGGAAGAGCACACGTC65550.16028101871730194No Hit
TGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACACGTC65160.15932740167230197No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA64030.15656435741371233No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG63930.15631984022268672No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC63620.15556183693050724No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT63410.15504835082935342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA61680.15081820342460997No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA59250.14487643568268713No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC57400.1403528676487129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGCCTGAAAGTCTGGATACCATTATGAAAGAGATCGGAAGAGCACAC56390.13788324401935403No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA55380.13541362038999513No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA53190.1300586939065338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG52590.12859159076038TruSeq Adapter, Index 4 (97% over 37bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA52490.12834707356935438No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA50280.12294324364768788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGCTGGCAAGTCCGGCAATGACCGAACTGGAAATGAGATCGGAAGAG48940.11966671328794443No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG48820.11937329265871366No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT47820.11692812074845735No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG46970.11484972462473947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC46840.11453185227640614No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG44780.10949479814127813No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA44560.10895686032102173No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA44090.10780762952320125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA43950.10746530545576538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA43830.10717188482653461No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC43550.10648723669166285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT42540.10401761306230396Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGCAGTTGGTGCAACCTTTACCGAAGATCGTCATCAGATCGGAAGAGC42440.10377309587127834No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA41880.10240379960153478No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA41820.10225708928691941No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAAGATCGGAA40950.1001297897249964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)