FastQCFastQC Report
Fri 14 Jul 2023
SRR9691472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3964825
Total Bases301.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT771431.9456848662929638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC490781.2378352134079058No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA416371.0501598431204402No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC368110.9284394645413101Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA311160.7848013468438078No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG288780.7283549715308999No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG276690.6978618224007365No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC234720.59200595234342No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC213160.5376277641510029No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA206660.5212335979519903Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA203700.5137679468829015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC174190.43933843233938447No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCACACGTC168590.4252142276140813No Hit
ATCTAATTACTGAAGAGACTACTCATGAGATCGGAAGAGCACACGTCTGA163320.41192234209580497Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG163110.4113926844186061Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG162970.4110395793004735No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG151510.3821354032019068No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTAGATCGGAAGA149910.3780999161375344No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG147310.3715422496579294No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT144030.3632695011759661No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC142500.3594105666706601No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA140770.3550471962823076No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG132860.33509675710781683No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC127770.3222588638842824No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACA125110.3155498666397634No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC123090.31045506422099334No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA121700.3069492348338199No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA111860.2821309893879301No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA106760.26926787437024335No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC106000.26735101801466643No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC97080.24485317763079076No Hit
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT96570.24356686612902206No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT96040.24223011103894876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA92090.23226750234877958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT90680.2287112293733015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAG87050.21955571809600677No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA86070.21708398226907868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG83170.20976966196490388No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCA74780.1886085766711015No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT73900.18638905878569673No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA73660.18578373572604087No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG73460.18527929984299432No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG72820.1836651050172454No Hit
ATCTAATTACTGAAGAGACTACTCATGTTAGATCGGAAGAGCACACGTCT71900.1813446999552313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC71890.181319478161079No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCA71450.18020971921837659No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA70330.17738487827331598No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC65080.16414343634334427No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG64430.16250401972344303TruSeq Adapter, Index 12 (97% over 37bp)
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAG62450.15751010448128228No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG62350.157257886539759No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62300.15713177756899738No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC61270.1545339327713077No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG61190.1543321584180891No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC58380.14724483426128518No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA57240.1443695497279199No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA56610.1427805766963233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA54370.13713089480620202Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT53620.1352392602447775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA53080.13387728336055185No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGA52480.13236397571141223No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA51870.1308254462681203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC51380.12958957835465626No Hit
ATGTTGTTATGAAAACAGATGCTGAGTAGATCGGAAGAGCACACGTCTGA51140.1289842552950004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAG50100.1263611887031584No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG49360.1244947759358862No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT48950.1234606823756408No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT47970.12098894654871274TruSeq Adapter, Index 1 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA46770.1179623312504335No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA46690.11776055689721489No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC46380.11697868127849274Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG45220.11405295315682282No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACACGT45150.11387640059775653No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA44000.11097589427023892No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA43470.10963913918016557No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC43410.10948780841525162No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC43220.1090085943263574Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA42910.10822671870763527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC42050.10605764441053514No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA41840.10552798673333628No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG40940.10325802525962685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40730.10272836758242797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA40710.10267792399412332No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA39860.10053407149117552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)