FastQCFastQC Report
Fri 14 Jul 2023
SRR9691473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100486
Total Bases311.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT795061.9389408962742465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC500171.2197822404466203No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA424851.0360966968305707No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC372700.9089166503677857Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA322080.7854678689306585No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG296910.7240849011556192No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG284510.6938445833006136No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC242110.590442206118982No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC216600.5282300683382409No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA211000.5145731505972707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA208760.5091103835008826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC177160.43204634767683636No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG172610.42095010201229804No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCACACGTC170470.41573120844699873No Hit
ATCTAATTACTGAAGAGACTACTCATGAGATCGGAAGAGCACACGTCTGA167790.40919539781382014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG166610.4063176901469728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG156390.3813938152697022No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG149960.3657127472206953No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTAGATCGGAAGA149930.3656395851613687No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC147140.35883551364399247No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT145150.3539824303753263No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA143920.3509827859429345No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG136420.332692271111278No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC131400.3204498198506226No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC128040.3122556692060405No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACA128000.31215811979360497No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA125250.3054515976886642No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA113620.27708910602304215No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA111600.2721628606950493No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC108700.26509052829347546No Hit
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT98630.24053246371283796No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT97700.23826443987371254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC97590.23799617898951492No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA93580.22821685039285589Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT93410.22780226539000498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG89770.21892526885837435No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA89330.21785222532158385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAG87240.21275526852182888No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCA76950.1876606821727961No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG76600.18680712481398545No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG75140.18324657126008967No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC73390.17897878446603646No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA73100.17827155122587907No Hit
ATCTAATTACTGAAGAGACTACTCATGTTAGATCGGAAGAGCACACGTCT72710.17732044445463294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCA72610.17707657092354417No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT72310.17634495033027792No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA71580.17456467355333002No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC66440.16202957405536808No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG66260.1615906016994083TruSeq Adapter, Index 12 (97% over 37bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG65450.1596152260975894No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG64590.15751791373022614No Hit
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAG63990.15605467254369362No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT63280.15432317047296343No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC62830.15322573958306407No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC61910.15098210309704752No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA57980.1413978733252595No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA56180.13700814976566192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA55390.13508154887006077No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA55330.13493522475140754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT54270.13235016532186672Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTTGTTATGAAAACAGATGCTGAGTAGATCGGAAGAGCACACGTCTGA54160.1320819044376691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGA53870.1313746711975117No Hit
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAG52900.12900909794595078No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA52160.12720443381589402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC51020.12442427556148222No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT50540.12325368261225621No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT50200.12242451260655444TruSeq Adapter, Index 1 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG49440.12057107377027991No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC48990.11947364288038052Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA48400.11803478904695687No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA47610.11610818815135572No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG47050.11474249637725868No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA45900.11193795076973802No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACACGT45510.11098684399849189No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA45000.10974308898993924No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA44510.10854810868760434Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC44300.10803597427231795No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC44070.10747506515081381No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC43990.10727996632594282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG43420.10588988719873692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA43100.10510949189925292No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC42870.1045485827777488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA41670.10162210040468374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)