FastQCFastQC Report
Fri 14 Jul 2023
SRR9691474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4129624
Total Bases313.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT800221.937755107971089Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC510601.2364321788133739No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA433021.048570039306242No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC380440.9212460989184488Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA327610.7933167765394622No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG298900.7237947086708136No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG290180.7026789848179883No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC240420.5821837532908565No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC220200.5332204578431353No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA211900.5131217757355149Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA209040.5061962057562626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC180360.4367467837265572No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG174710.4230651507255866No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCACACGTC173880.4210552825148246No Hit
ATCTAATTACTGAAGAGACTACTCATGAGATCGGAAGAGCACACGTCTGA171520.415340476517959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG168430.4078579551068088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG159460.38613684926279No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTAGATCGGAAGA152680.36971888966162536No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG151910.3678543131287497No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT147100.356206763618189No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA145260.3517511521630057No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC144700.3503950965027325No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG139290.3372946302133075No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC132320.3204165802988359No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC129740.3141690381497202No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACA129560.3137331631160609No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA125470.30382911374013716No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA115160.2788631604233218No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA114170.276465847738196No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC108460.26263892305933906No Hit
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT99700.24142633808792277No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT98860.23939225459751298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC97500.23609897656542098No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA93930.2274541217311794Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT92080.22297429499634833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAG92020.2228290033184619No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA90380.2188576974562333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG89620.2170173362030054No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG77340.18728097279558623No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCA76940.18631236160967682No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG76360.18490787539010817No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA73860.1788540554781743No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT73780.1786603332409924No Hit
ATCTAATTACTGAAGAGACTACTCATGTTAGATCGGAAGAGCACACGTCT73500.17798230541085583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA73430.17781279845332168No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCA73380.177691722055083No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC69940.16936166585626197No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC68550.16599574198522674No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG67060.16238766531771415No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG64720.15672128988014405No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG64650.1565517829226099TruSeq Adapter, Index 12 (97% over 37bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC63800.1544934841525524No Hit
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAG63320.1533311507294611No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT63300.15328272017016562No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC59710.14458943477662856No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA57700.13972216356743375Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA57460.13914099685588807No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA55680.13483067707859117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT55530.13446744788387513Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA55080.13337776029972703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAG54740.13255444079170403No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA54450.13185219768191972No Hit
ATGTTGTTATGAAAACAGATGCTGAGTAGATCGGAAGAGCACACGTCTGA54230.13131946152966953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGA54090.13098044761460123No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT51850.1255562249735085No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC51570.12487819714337188No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT50750.12289254421225758TruSeq Adapter, Index 1 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG50050.12119747463691609No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA49580.12005935649347253No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACACGT47850.1158701131144143No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC47740.1156037450382892Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG47510.11504679360639129No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA47450.11490150192850487No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA46050.11151136277782189No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA45070.10913826537234383No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC44650.10812122362713894No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA44540.10785485555101385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC44490.10773377915277516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC44470.1076853485934797No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC43350.10497323727293331Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA43190.10458579279856955No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA42380.102624355147103No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG41730.10105036197000017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)